Probiotic agent for fermenting silage and production method thereof

A technology of silage and probiotics, applied in the field of animal husbandry, can solve the problems of high cost and unclear dosage, and achieve the effects of low production cost, high nutrition and simple preparation method

Inactive Publication Date: 2017-06-30
广西山水牛畜牧业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the amount of various silage additives used in different silage crops is not clear

Method used

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  • Probiotic agent for fermenting silage and production method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 probiotics and silage preparation:

[0040] Preparation of probiotics:

[0041] (1) Preparation of medium:

[0042]Take 4L of rice washing water, add 100g of potato powder and 40g of urea, stir and mix evenly, take 100g of beef liver and grind it into fine powder, add to the mixture, stir and mix evenly, and sterilize at 120-123°C for 30 minutes;

[0043] (2) Inoculation and cultivation of probiotics:

[0044] Cool the medium prepared in step (1) to room temperature, and inoculate Bacillus subtilis 2×10 10 CFU / G, Lactobacillus 8×10 9 CFU / G, Lactobacillus sake 4×10 10 CFU / G, Lactobacillus acidophilus 3×10 10 CFU / G, the inoculum size is 1g, stir and mix to disperse the strains evenly in the culture medium, ferment aerobically at 25°C for 50h, then anaerobically ferment at 32°C for 30h, and separate in a sterile room Pack in strain bag, every bag 200ml promptly obtains the probiotic agent for fermenting silage of the present invention;...

Embodiment 2

[0046] The preparation of embodiment 2 probiotics and silage preparation:

[0047] Preparation of probiotics:

[0048] (1) Preparation of medium:

[0049] Take 4L of rice washing water, add 100g of potato powder and 40g of urea, stir and mix evenly, take 100g of beef liver and grind it into fine powder, add to the mixture, stir and mix evenly, and sterilize at 120-123°C for 30 minutes;

[0050] (2) Inoculation and cultivation of probiotics:

[0051] The medium prepared in step (1) was cooled to room temperature, and inoculated with Aspergillus niger 2 × 10 10 CFU / G, Lactobacillus 8×10 9 CFU / G, Lactobacillus sake 4×10 10 CFU / G, Lactobacillus acidophilus 3×10 10 CFU / G, the inoculum size is 1g, stir and mix to disperse the strains evenly in the culture medium, ferment aerobically at 25°C for 50h, then anaerobically ferment at 32°C for 30h, and separate in a sterile room Pack in strain bag, every bag 200ml promptly obtains the probiotic agent for fermenting silage of the pre...

Embodiment 3

[0053] The preparation of embodiment 3 probiotics and silage preparation:

[0054] Preparation of probiotics:

[0055] (1) Preparation of medium:

[0056] Take 4L of rice washing water, add 100g of potato powder and 40g of urea, stir and mix evenly, take 100g of beef liver and grind it into fine powder, add to the mixture, stir and mix evenly, and sterilize at 120-123°C for 30 minutes;

[0057] (2) Inoculation and cultivation of probiotics:

[0058] The medium prepared in step (1) was cooled to room temperature, and inoculated with Aspergillus niger 2 × 10 10 CFU / G, Bacillus subtilis 2×10 10 CFU / G, Lactobacillus 8×10 9 CFU / G, Lactobacillus sake 4×10 10 CFU / G, Lactobacillus acidophilus 3×10 10 CFU / G, the inoculum size is 1g, stir and mix to disperse the strains evenly in the culture medium, ferment aerobically at 25°C for 50h, then anaerobically ferment at 32°C for 30h, and separate in a sterile room Pack in strain bag, every bag 200ml promptly obtains the probiotic agen...

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Abstract

The invention provides a probiotic agent for fermenting silage and a production method thereof; the probiotic agent is composed of aspergillus niger, bacillus subtilis, bacillus laevolacticus, lactobacillus sake and lactobacillus acidophilus; the preparation method is simple, and the cost is low; the probiotic agent has significant effect on fermentation of the silage; the silage after fermentation is rich in fermentation cellulose lactic acid, calcium lactate, small peptides, amino acids, vitamins and probiotics, has the characteristics of high nutrition, easy digestion, good palatability and the like, can significantly reduce animal digestive system diseases, and is quite suitable for animal growth.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a probiotic agent for fermenting silage and a production method. Background technique [0002] With the rapid development of the global economy, people's living standards are constantly improving, especially in developed countries and emerging developing countries, the demand for high-grade beef milk consumption is increasing rapidly. Because of its high protein content, low fat content, and comprehensive nutrition, beef is widely used in The proportion of meat consumption is increasing, while the consumption demand of milk and dairy products is also increasing. High-quality beef and milk products have become the desire and pursuit of modern people. Therefore, how to improve breeding technology, guarantee the supply of feed and improve the quality of feed, and then improve the quality of beef has become an urgent problem for those in the technical field. ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A23K30/18C12R1/685C12R1/125C12R1/07C12R1/225C12R1/23
CPCC12N1/14C12N1/20A23V2400/169
Inventor 杨远澄周贵金谭春萍杨远广陆海龙
Owner 广西山水牛畜牧业有限责任公司
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