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Method for extracting Afzelin from Chinese redbud leaves

A technology of alfodzin and Bauhinia leaves is applied in the field of extracting alfondoside to achieve the effects of rich raw material sources, recyclable solvent and wide biological activity.

Active Publication Date: 2017-07-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that afudouside has broad prospects. Although afudouside has a relatively high content in Bauhinia leaves, there is no report of extracting a large amount of afudouside from Bauhinia plants.

Method used

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  • Method for extracting Afzelin from Chinese redbud leaves

Examples

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Effect test

Embodiment 1

[0020] A method for extracting afrudin from Bauhinia leaves, comprising the following steps:

[0021] (1) 4.5 kg of dried Bauhinia leaves were used as raw materials, crushed and extracted with 70% ethanol (the amount of 70% ethanol used was 25L) at 50°C for 3 times, each time for 3 hours, the extracts were combined, and the solvent was recovered under reduced pressure to obtain the extract Extract: add water to dissolve the extract extract, load the D-101 macroporous resin column, and sequentially use 20% ethanol, 40% ethanol, 60% ethanol and 90% ethanol for gradient elution to obtain the corresponding elution fraction;

[0022] (2) Mix the eluted part of 40% ethanol obtained in step (1) with 60-mesh silica gel according to the weight ratio of 1:30 and load it into the column. Dichloromethane and methanol are eluted according to the volume ratio of 10:1, TLC detection, and combined At the same point, the solvent is recovered under reduced pressure to obtain the crude product o...

Embodiment 2

[0031] A method for extracting afrudin from Bauhinia leaves, comprising the following steps:

[0032] (1) Use dried Bauhinia leaves as raw materials, crush them and extract them twice with 65% ethanol at 55°C for 3.5 hours each time, combine the extracts, recover the solvent under reduced pressure to obtain the extract extract; dissolve the extract extract in water, After the D-101 macroporous resin column is loaded, it is sequentially eluted with 20% ethanol, 40% ethanol, 60% ethanol and 90% ethanol to obtain the corresponding eluted fraction;

[0033] (2) Mix the eluted part of 40% ethanol obtained in step (1) with 80-mesh silica gel according to the weight ratio of 1:29 and load it into the column. Dichloromethane and methanol are eluted according to the volume ratio of 9:1, TLC detection, and combined At the same point, the solvent is recovered under reduced pressure to obtain the crude product of afudouside;

[0034] (3) Dissolving the crude afruddin in step (2) with met...

Embodiment 3

[0037] A method for extracting afrudin from Bauhinia leaves, comprising the following steps:

[0038] (1) Use dried Bauhinia leaves as raw materials, crush them and extract them with 75% ethanol at 45°C for 4 times, each time for 2.5 hours, combine the extracts, recover the solvent under reduced pressure to obtain the extract extract; dissolve the extract extract in water, After the D-101 macroporous resin column is loaded, it is sequentially eluted with 20% ethanol, 40% ethanol, 60% ethanol and 90% ethanol to obtain the corresponding eluted fraction;

[0039] (2) Mix the eluted part of 40% ethanol obtained in step (1) with 100-mesh silica gel according to the weight ratio of 1:31 and load it into the column. Dichloromethane and methanol are eluted according to the volume ratio of 10:1, TLC detection, and combined At the same point, the solvent is recovered under reduced pressure to obtain the crude product of afudouside;

[0040] (3) Dissolving the crude afruddin in step (2)...

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Abstract

The invention discloses a method for extracting Afzelin from Chinese redbud leaves. The method comprises crushing dried Chinese redbud leaves as raw materials, carrying out extraction on the Chinese redbud leaf powder through 70% ethanol at 50 DEG C, carrying out vacuum recovery in a solvent in the extract to obtain extract, dissolving the extract in water, loading the solution as a sample to a D-101 macroporous resin column, carrying out gradient elution through different concentrations of ethanol solutions to obtain corresponding eluted parts, mixing the eluted part of 40% ethanol and silica gel, filling the column with the mixture, carrying out elution through dichloromethane and methanol, carrying out thin layer detection, merging the same points, carrying out solvent vacuum recovery to obtain a crude product, dissolving the crude product, treating the solution through a LH-20 gel chromatographic column, and carrying out methanol elution to obtain pure Afzelin. The method is conducive to development and use of Chinese redbud leaves as medical plants and sustainable utilization of plants. The method has simple processes, easily produces an Afzelin monomer, realizes recovery and recycle of the solvent, realizes a large extraction amount and can be industrialized.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting afudouside from bauhinia leaves. Background technique [0002] Bauhinia ( Mitragyna rotundifolia Kuntze.) is the genus Bauhinia (Rubiaceae) of the leguminous family (Rubiaceae) Mitragyna L.) Plants. The bark and wood can be used as medicine, which has the functions of promoting blood circulation and promoting qi, clearing away heat and detoxification, reducing swelling and relieving pain. Bauhinia can be used to treat rheumatic bone pain and malnutrition in the nose; Bauhinia fruit can treat cough. [0003] The natural compound afudouside involved in the present invention is obtained for the first time from the plant Bauhinia genus Bauhinia, and belongs to a kind of flavonol compounds. Azoside is rich in sources and exists in a variety of plants. Modern pharmacological studies have shown that it has inhibitory effects on ovalbumin-...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 康文艺何楠呼谧允王鹏禹
Owner HENAN UNIVERSITY
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