A method for identifying populations of rice stem borer and wild rice stem
A kind of technology of Diploid and population, applied in the direction of instruments, scanning probe technology, etc., can solve the problems of complicated identification work, difficult to widely use, time-consuming and other problems, and achieve simple, fast and reliable methods, reliable experimental results, and time saving. Effect
Active Publication Date: 2020-04-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology
At the same time, the above techniques need to identify populations through large sample size and long-term observation, so it is difficult to be widely used
In addition, the identification of different populations of Chilo suppressalis in the above techniques is cumbersome and complicated, which not only consumes a lot of time, but also many external uncertain factors that appear in the detection process are likely to cause certain experimental errors that cannot be ruled out.
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Embodiment 1
[0035] Test period: July 15, 2015 to July 31, 2016
[0036] Test location: Hangzhou City, Zhejiang Province, Plant Protection Engineering Laboratory, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences.
[0037] The number of test insects: 300.
[0038] Experimental results: This method was used to assist in the identification of Zizania stem borer populations and rice populations.
[0039] The collection, sample processing and observation of the larvae of rice stem borer and wild rice stem populations are carried out in the following ways:
[0040] In May 2015, the overwintering larvae of Chilo borer were collected from the main rice production area (Sanqi Town, Yuyao City) and the wild rice stem production area (Xinjian Town, Jinyun County) in Zhejiang Province, and brought back to the laboratory for rearing. The indoor temperature is controlled by air conditioning at 28±1°C, the photoperiod is 16 hours of light and 8 hours of darkness...
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The invention discloses a method for identifying a rice population and a water bamboo population of chilo suppressalis walkers. The method comprises the steps of acquiring a chilo suppressalis walker larva to be identified, cutting the head of the chilo suppressalis walker larva, observing and shooting the head of the chilo suppressalis walker larva by using a scanning electron microscope after pretreatment, calculating the number of various sensilla on a tentacle and a mouthpart of the head, and identifying the population of the chilo suppressalis walker according to the following rules: (1) a tentacle flagellum of the water bamboo population is provided with a styloconic sensillum and two conical sensilla, and a tentacle flagellum of the rice population is provided with a styloconic sensillum and a conical sensillum; and a maxillary palp of the water bamboo population is provided with five conical sensilla and three cylindrical sensilla, and a maxillary palp of the rice population is provided with four conical sensilla and four cylindrical sensilla. Different populations of the chilo suppressalis walkers can be identified and differentiated through simply accounting the number of sensilla on the tentacle and the mouthpart in a short time. The method is simple, quick and reliable.
Description
technical field [0001] The invention belongs to the field of insect population distinction and is a new method for indoor and field auxiliary identification of different host populations of Chilo suppressalis. Background technique [0002] Various sensilla (thorn-shaped, cone-shaped, peg-cone-shaped, plate-shaped, etc.) concentratedly distributed on the antennae and mouthparts of insects are structures that respond to various external stimuli and regulate the behavior of insects together with the nervous system. Sensilla are closely related to the physiological behavior of insects, and play a very important role in food discrimination, host selection, courtship and other behaviors. The type, number, distribution and function of sensilla in different species of insects are not the same, which provides a new idea for ultrafine morphological classification. Insect sensilla have obvious differences at the genus level and above, and there are also differences between different d...
Claims
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IPC IPC(8): G01Q30/02G01Q30/20
CPCG01Q30/02G01Q30/20
Inventor 钟海英陈建明张珏锋李芳
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES