Neural stem cell preparation for treating Parkinson's disease through nasal delivery

A technology for neural stem cells and Parkinson's disease, applied in the field of neural stem cell preparations and their preparation, can solve the problem of irreversible degeneration of DA neurons, and achieve the effects of good curative effect and safety, repair of nerves and tissues, and stable performance.

Inactive Publication Date: 2017-07-07
SHANGHAI ANGECON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods belong to symptomatic treatment, but they cannot reverse the degeneration of DA neurons. Therefore, finding a new technology and method that can replace the degenerated dopaminergic neurons to restore the function of dopamine neurotransmitters has become an urgent need

Method used

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  • Neural stem cell preparation for treating Parkinson's disease through nasal delivery
  • Neural stem cell preparation for treating Parkinson's disease through nasal delivery
  • Neural stem cell preparation for treating Parkinson's disease through nasal delivery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Acquisition of Neural Stem Cell Seed Bank Cells

[0032] 1. Induction of primary neural stem cells from embryonic stem cells (ESCs)

[0033] Use a cell scraper to pick ESCs cell clones, blow them gently, and then resuspend them with neural stem cell induction medium, transfer them to an ultra-low adsorption culture dish for suspension culture for about 3 days, collect the formed cell balls, blow them gently, and then use neural stem cell The stem cell induction medium was resuspended, suspended and cultured in an ultra-low adsorption culture dish for 3 days, and the formed cell spheres were collected; the culture dish coated with Laminin was washed twice with DPBS without Ca and Mg one day in advance, and the collected The cell balls were resuspended with the complete medium of neural stem cells, inoculated in the coated culture dish for adherent culture, and the medium was changed every 2-3 days until obvious neural-like colonies were formed, and then the fo...

Embodiment 2

[0044] Example 2: Acquisition of Neural Stem Cell Master Cell Bank Cells

[0045] 1. Coating of culture flasks: add an appropriate amount of laminin Laminin solution to the cell culture flasks, put them in a 37°C incubator for coating for 2 hours, and then wash them once with DPBS without Ca and Mg for later use;

[0046] 2. After digesting and counting the amplified and purified P5 neural stem cells, resuspend them in complete serum-free medium, and adjust the density to 1x10 5 cells / ml, inoculated in coated culture flasks, placed at 35°C 5% CO 2 The adherent culture is carried out in the incubator; the cell culture medium is changed every 48 hours, and the cells can be digested and passaged after continuous culture for about 7 days until the P8 generation. The cells obtained during this period are stored in the main cell bank.

Embodiment 3

[0047] Example 3: Acquisition of Neural Stem Cell Working Cell Bank Cells

[0048] Resuspend P8 passage cells in serum-free complete medium, and adjust the density to 1x10 5 cells / ml, inoculated in coated culture flasks, placed at 35°C 5% CO 2 Adhesive culture is carried out in the incubator. During the culture process, samples are taken to detect cell characteristics and heterologous substances. After passing the test, the cells are frozen and transferred to the working cell bank for later use.

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Abstract

The invention belongs to the field of biological medicine and specifically relates to a neural stem cell preparation for treating a Parkinson's disease through nasal delivery as well as a preparation method and application thereof. The preparation method comprises the following steps: cultivation and purification of seed cells; cultivation of P6-P8 generation cells; cultivation of a working cell repository; preparation of a preparation for treating the Parkinson's disease. According to the preparation as well as the preparation method and the application thereof, after the preparation is dropped or injected into a nasal cavity, the preparation can quickly form a solid membrane shape under a body temperature condition and can be adsorbed on the nasal cavity for slow absorption, and the preparation cannot enter an oral cavity or lungs along with breathing, so that the preparation can be used by patients and doctors conveniently and fast; in addition, test data indicate that the preparation has better curative effect and safety compared with a traumatic corpus striatum delivery. The preparation can be used for treating the Parkinson's disease, and an immunosuppressive agent is not needed.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a neural stem cell preparation for treating Parkinson's disease administered through the nasal cavity, a preparation method and application thereof. Background technique [0002] Parkinson's disease (Parkinson's disease, PD) is a relatively common clinical refractory disease, characterized by the degeneration and loss of dopaminergic neurons in the substantia nigra and the formation of Lewy bodies. Stiffness and abnormal posture and gait greatly affect the patient's quality of life. Beginning in the late 1960s, dopamine replacement therapy with oral levodopa to relieve motor symptoms in PD is one of the successes of translational research in modern neuroscience. Currently, levodopa remains the gold standard for the treatment of symptomatic PD. Currently, the most effective method of PD treatment is the use of levodopa replacement therapy, but it still cannot effectively prevent or sl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61K9/00C12N5/0797A61P25/16
Inventor 赵雄飞郑佳威黄倩莹
Owner SHANGHAI ANGECON BIOTECH
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