Dawuzui hemerocallis citrina baroni purification and rejuvenation method

Abstract

The invention discloses a Dawuzui hemerocallis citrina baroni purification and rejuvenation method, which comprises: selecting a hemerocallis citrina baroni parent strain, peeling an explant, disinfecting the explant, carrying out primary culture, carrying out proliferation culture, carrying out seedling reinforcing culture, carrying out rooting culture, carrying out seedling exercising transplantation on tissue culture seedling, and carrying out seedling exercising on plug seedling. According to the present invention, the operation is simple, the cost is low, the rapid breeding of the Dawuzui hemerocallis citrina baroni within the short time can be effectively promoted, and the original seed property, the stability and the uniformity of the Dawuzui hemerocallis citrina baroni are maintained during the breeding property; and with the application of the method of the present invention to perform tissue culture, the plug seedling grows to achieve 10-15 cm after the culture in the plug for 2-3 months, the seedling is vigorous, upright and fresh, the number of the leaves is more than eight, the shape is complete, the size of the leaf is coordinated, the leaf color has the characteristic of the original variety, the leaf is shiny, the root system is complete and developed, the plug hole is filled with the root system, the root can be easily pulled out while the matrix is not scattered, the heights of more than 90% of the seedlings in the same batch meet the standard, and the obtained Dawuzui hemerocallis citrina baroni is suitable for the large area planting in the Suqian area.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for purifying and rejuvenating day lilies. Background technique [0002] Day lily, also known as "day lily", belongs to the genus Hemerocallis in the Liliaceae family. It is rich in nutrients. It is also a treasure among vegetarian dishes, along with shiitake mushrooms, fungus, and winter bamboo shoots. Mainly distributed in Jiangsu, Hunan, Hubei, Sichuan and other places, Suqian is one of the main producing areas of day lilies in Jiangsu Province, especially "Dawuzui" with long and thick flower buds, bright yellow color, firm flesh, sweet and crispy taste, and high quality It is well-known at home and abroad; however, due to the use of traditional cultivation techniques, the purification and rejuvenation work of Dawuzui has been neglected, resulting in serious mixing, degradation of species, and decline in quality and yield; in response to this problem, the...

AI Technical Summary

Problems solved by technology

Mainly distributed in Jiangsu, Hunan, Hubei, Sichuan and other places, Suqian is one of the main producing areas of day lilies in Jiangsu Province, especially "Dawuzui" with long and thick flower buds, bright yellow color, firm flesh, sweet and crispy taste, and high quality It is well-known at home and abroad; however, due to the use of traditional cultivation techniques, the purification and rejuvenation work of Dawuzui has been neglected, resulting in serious mixing, degradation of species, and decline in quality and yield; in response to this problem, the present invention provides a kind of Dawuzui Tissue culture rapid propagation method of day lily lily, including steps such as the establishment of explants, starting culture, subculture, rooting culture, seedling hardening and transplanting