Unlock instant, AI-driven research and patent intelligence for your innovation.
High polymer material for complexing copper ions and anti-tumor application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A polymer material and copper-complexing technology, which is applied in the field of polymer materials complexed with copper ions, can solve problems such as limited development and clinical application, and high toxicity
Active Publication Date: 2017-07-14
TIANJIN MEDICAL UNIV
View PDF2 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
At present, the existing tumor vascular inhibitors, such as CA4, etc., have limited their development and clinical application because of their high toxicity.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0045] Example 1: Synthesis and structural characterization of material 1.
[0046] 1) Take 0.03mmol of polyglutamic acid (γ-PGA, Mw=100,000) in 60mL of water, add 3mmol of Na 2 CO 3 , dropwise added anhydrousethanol containing 3mmol di-tert-butyl dicarbonate (Boc), and reacted in ice bath for 24h. After the reaction was completed, it was precipitated with 500 mL of absolute ethanol, washed, and dried to obtain intermediate product 1, Boc-PGA.
[0047] 2) Dissolve 0.003mmol of Boc-PGA in 2mL of water, add 1.8mmol of triethylenetetramine (TETA), add 1mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Aqueous solution of hydrochloride (EDC) and 1.8mmol N-hydroxysuccinimide (NHS), adjust the pH to 6.0 with dilute hydrochloric acid, and react at room temperature for 24h; after the reaction, precipitate and wash with 40mL acetone, and dry to obtain the intermediate product 2, Boc- PGA-TETA.
[0048] 3) Dissolve 0.003mmol of Boc-PGA-TETA in 3mL of water, add 0.615mmol of sodiu...
Embodiment 2
[0053] Example 2: Preparation of material 1 and its encapsulated R848 nanospheres.
[0054] Dissolve 1 mg of material 1 in 400 μL of pH 3.0 glycine-HCl buffer, add 100 μL of pH 3.0 glycine-HCl buffer containing 0.4 mg R848 or without R848, vortex to dissolve, and add to 500 μL pH7.65 while stirring In the borate buffer solution, the final pH is 7.2, the nanosphere structure is observed by transmission electron microscopy, see image 3 .
Embodiment 3
[0055] Example 3: Ultraviolet absorption detection of the complexing ability of materials 1 and 2 to copper ions.
[0056] Take three 5mL centrifuge tubes, add 2mL ultrapure water to each tube, add 200μL of 500μg / mL γ-PGA, material 1 or material 2 solution respectively, and detect the ultraviolet absorption peak in the 190nm~400nm band. Take another 3 5mL centrifuge tubes, add 2mL of CuSO with a concentration of 100μM to each tube 4 200 μL of 500 μg / mL γ-PGA, material 1 or material 2 solutions were added to the solution, and the ultraviolet absorption peak was detected in the 190nm-400nm band (U-3310 spectrophotometer, Hitachi High-Technologies Corporation, Tokyo, Japan). The ultraviolet absorption peaks of materials 1 and 2 shifted to the right after complexing copper ions, see Figure 4 .
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to a high polymer material for complexing copper ions and anti-tumor application thereof. Shown as a formula I, A is selected from polyglutamic acid or hyaluromic acid, B is selected from triethylenetetramine or diethylenetriamine, and C is selected from poly-L-histidine or polylactic acid. The material is extremely small in toxicity, and can remove copper ions in a tumor micro-environment by complexing to inhibit tumor angiogenesis so as to further inhibit tumor metastasis; in addition, the material can be formed into a nanosphere with the diameter of 200 to 280nm when pH reaches 7.0-8.0; the nanosphere can load immunostumulating agent such as R848, immunologic cells are activated in the tumor micro-environment to kill tumor cells, so that the combined treatment of tumor immunotherapy and anti-tumor angiogenesis is realized. The formula (1) is shown in the specification.
Description
technical field [0001] The invention relates to the field of pharmaceutical polymer materials, in particular to a polymer material for complexing copper ions and its antitumor application. Background technique [0002] Tumor metastasis is the main cause of poor prognosis and high mortality in cancer patients, and tumor metastasis depends on tumor angiogenesis. Angiogenesis plays an important role in tumor development and metastasis. Studies have shown that in benign tumors, angiogenesis is rare and blood vessels grow slowly; while in most malignant tumors, angiogenesis is dense and grows rapidly. The proliferation and migration of vascular endothelial cells are two important conditions for the formation of tumor blood vessels. At present, the existing tumor vascular inhibitors, such as CA4, etc., have limited their development and clinical application because of their high toxicity. Bevacizumab, as a VEGFR inhibitor, also does not bring much benefit to the majority of can...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More