Device and method for extracting and purifying nucleic acid
A purification device and nucleic acid technology, which is applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of accelerating extraction speed, numerous steps, and high cost, so as to speed up extraction and avoid Effect of cross-contamination and simplified nucleic acid extraction process
Pending Publication Date: 2017-07-14
苏州天隆生物科技有限公司
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Problems solved by technology
These steps are numerous and complicated. The traditional method is manually processed by professionals in the laboratory. However, these manual processing methods are inefficient, expensive, and have operational errors
With the continuous emergence of nucleic acid extraction equipment with different degrees of automation, laboratory personnel can use nucleic acid extraction equipment to process multiple different samples at the same time, but how to package, integrate, and integrate the reagents necessary for nucleic acid extraction in a closed system , simplify the nucleic acid extraction process, speed up the extraction speed, avoid cross-contamination between samples at the same time, and realize complex processing devices and methods for automatic implementation of nucleic acid extraction from one step to the next step, which are still expected by the market and urgently need to be developed of
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Embodiment 2
[0081] Figure 7 It shows the schematic plan view of surface A of Embodiment 2 of the device of the present invention. The difference between this embodiment and the device of Embodiment 1 is that this embodiment includes five reagent chambers, which are respectively the first reagent chambers for storing cell lysate 4. The second reagent chamber 7 for storing the washing liquid 1, the third reagent chamber 10 for storing the washing liquid 2, the fourth reagent chamber 13' for storing the washing liquid 3, and the nucleic acid washing chamber for storing Deliquified fifth reagent chamber 13 . The number of washing liquid chambers of the device of the present invention can be increased or decreased according to sample properties, extraction time, and downstream experimental requirements.
[0082] The nucleic acid extraction and purification device and method provided by the present invention are applicable to various liquid samples, including but not limited to biological sam...
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The invention discloses a device and a method for extracting and purifying nucleic acid. Reagents which are essential for nucleic acid extraction are packaged, combined and integrated in an enclosed system; under the action of external force applied by a matched instrument, nucleic acid extraction, purification and subsequent detection and analysis processes are realized in sequence. By adopting the device and the method, a nucleic acid extraction flow is simplified, the extraction speed is increased, meanwhile cross infection among samples is avoided, and automatically-implemented complicated treatment from one step to a next step of nucleic acid extraction is realized.
Description
technical field [0001] The invention relates to the field of nucleic acid extraction and purification, in particular to a nucleic acid extraction and purification device and method based on a three-dimensional double-sided disk device and driven by centrifugal force. Background technique [0002] Nucleic acid is a biomacromolecular compound synthesized by many nucleotides. It is the carrier of the genetic information of organisms and one of the most basic substances of life. Therefore, whether it is to conduct research on nucleic acid sequence and structure, or to explore gene function and expression, or to carry out genetic engineering or protein engineering, it is first necessary to separate and purify nucleic acid; at the same time, the quality of nucleic acid extraction products will directly affect related to the success or failure of subsequent experiments. Therefore, the extraction and purification of nucleic acid is the most important and basic operation in molecula...
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IPC IPC(8): C12M1/00
CPCC12N15/1003
Inventor 苗保刚李明彭年才李政田春孙瑶
Owner 苏州天隆生物科技有限公司