Theaflavin product rich in tea catechin
A technology of tea catechins and theaflavins, applied in the field of theaflavins rich in tea catechins, can solve the problems of ineffective synthesis of diester-type theaflavins and excessive consumption of tea catechins , to achieve the effect of increasing the conversion generation rate and reducing the loss rate
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Embodiment 1
[0038]Accurately weigh 0.7257 g of crushed and ground tender stems and place them in a 100 mL flat-bottomed Erlenmeyer flask, add 20 mL of water-saturated ethyl acetate, shake at room temperature (15 °C) for 5 min, and filter quickly with filter paper under reduced pressure to obtain the filtrate. After fully concentrating the filtrate at a vacuum degree of -0.07 MPa to -0.1 MPa and a temperature below 50 °C, add 20 mL of 5% ethanol aqueous solution, fully oscillate to extract the target substance and set the volume in a 25 mL volumetric flask, shake well before adding 10 μL of the sample was analyzed by HPLC (results in figure 1 Example-1 and Table 1), the rest of the solution was concentrated and dried to obtain theaflavin products rich in tea catechins.
[0039] Table 1 The integral area of each component in the HPLC analysis diagram
[0040] Element caffeine EC EGCG ECG TF TF-3-G TF-3′-G + TF-3, 3′-G Integral area 2238262 157493 1370645 ...
Embodiment 2
[0042] Accurately weigh 0.7023 g of crushed and ground tender stems and place them in a 100 mL flat-bottomed Erlenmeyer flask, add 20 mL of water-saturated ethyl acetate, shake in a water bath (35 °C) for 120 min, and quickly filter with filter paper under reduced pressure to obtain the filtrate. After fully concentrating the filtrate at a vacuum degree of -0.07 MPa to -0.1 MPa and a temperature below 50 °C, add 20 mL of 5% ethanol aqueous solution, fully oscillate to extract the target substance and set the volume in a 25 mL volumetric flask, shake well before adding 10 μL of the sample was analyzed by HPLC (results in figure 1 Example-2 and Table 2), the rest of the solution was concentrated and dried to obtain a theaflavin product rich in tea catechins.
[0043] Table 2 The integral area of each component in the HPLC analysis diagram
[0044] Element caffeine EC EGCG ECG TF TF-3-G TF-3′-G + TF-3, 3′-G Integral area 2076222 43586 522939 3...
Embodiment 3
[0046] Accurately weigh 0.6411 g of crushed and ground tender stems and 0.2170 g of crushed and ground tea tree leaves into a 100 mL flat-bottomed Erlenmeyer flask, add 20 mL of water-saturated ethyl acetate, shake in a water bath (35 °C) for 120 min, and quickly Filter the filter paper under reduced pressure to obtain the filtrate. After fully concentrating the filtrate at a vacuum degree of -0.07 MPa to -0.1 MPa and a temperature below 50 °C, add 20 mL of 5% ethanol aqueous solution, shake fully to extract the target substance and set the volume to 25 mL volumetric flask, shake well and inject 10 μL for HPLC analysis (results in figure 1 Example-3 and Table 3), the rest of the solution was concentrated and dried to obtain a theaflavin product rich in tea catechins.
[0047] Table 3 Integral area of each component in the HPLC analysis diagram
[0048] Element caffeine EC EGCG ECG TF TF-3-G TF-3′-G + TF-3, 3′-G Integral area 2749060 not dete...
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