Method and probe for detecting miRNA (Ribonucleic Acid) and/or target molecule with aptamer

A nucleic acid aptamer and probe technology, applied in biochemical equipment and methods, DNA/RNA fragments, DNA preparation, etc., can solve problems such as high cost and complicated operation
CN106957908AActive Publication Date: 2017-07-18LINYI UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LINYI UNIVERSITY
Publication Date
2017-07-18

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Abstract

The invention discloses a method and probe for detecting miRNA (Ribonucleic Acid) and / or a target molecule with an aptamer. The probe comprises a gold nanoparticle-H1 compound, a gold nanoparticle-H2 compound and a target recognition compound, wherein the gold nanoparticle-H1 compound and the gold nanoparticle-H2 compound are respectively formed by bonding neck ring DNA (Deoxyribonucleic Acid) H1 and H2 to gold nanoparticles; and the target recognition compound comprises at least one of a carboxyl magnetic microsphere-DNA / 2 compound for recognizing miRNA, and DNA3 for recognizing target molecules. The method disclosed by the invention does not need chemical markers, shows visible color change with naked eyes, is simple in operation and wide in application range and can be applied to detection on target molecules with the aptamer.
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Description

technical field

[0001] The invention relates to the technical field of nucleic acid hybridization detection, in particular to a detection method and a detection probe for miRNA and / or target molecules with nucleic acid aptamers. Background technique

[0002] miRNA is a class of endogenous non-coding small molecules (18-22nt), which play an important regulatory role in gene expression or reverse transcription. It is associated with a wide range of biological processes such as cell proliferation, apoptosis and death. Aberrant expression of miRNAs has been implicated in various diseases, especially human cancers, neurological diseases, viral infections and diabetes. The obvious regulatory role of miRNA in physiological and pathological processes makes miRNA be used in the diagnosis of clinical diseases, gene therapy and the discovery of new anticancer drugs. For example, miRNA-203 is overexpressed in various malignancies, including breast cancer, cervical cancer, leukemia and...

Claims

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