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Photinia serrulata tissue culture seedling rooting medium and culture method

The technology of rooting medium and heather group is applied in the field of rooting medium and culture of red-leaf heather tissue culture seedlings, which can solve the problems of callus swelling, easy occurrence of callus, low root vitality and the like, and achieves root development promotion and propagation improvement. , the effect of robust growth

Inactive Publication Date: 2017-07-21
HEFEI HUAGAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problems of Photinia fragrans tissue culture factory planting have not been completely solved. When MS medium is used for rooting culture of Photinia fragrans tissue culture seedlings, the following problems often occur: the callus at the base of the rooted plantlets in tissue culture is enlarged, the number of roots is small, and the rooting rate is low , low root activity, prone to callus and vitrification after multiple subcultures

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] One aspect of this embodiment provides a rooting medium for Photinia fragrans tissue culture seedlings. The rooting medium for Photinia fragrans tissue culture seedlings takes MS medium as the basic medium, and adds 19 g / L of sucrose and 1.5 g of activated carbon to the MS medium. g / L, organic fertilizer component 1.7g / L, bactericidal powder 1.5ml / L, inorganic salt composition 1.8g / L, heat with slow fire and keep stirring to dissolve the above components, add 1mol / L hydrochloric acid to adjust pH value, make the pH value of the rooting medium between 5.4-6.1, then add 3-6g of agar, stir and put it into a pressure sterilizer, and sterilize at 121°C for 20-30min.

[0040] The organic fertilizer component formula is as follows: 3000g of wheat bran, 500g of lactic acid bacteria, 500g of corn steep liquor, 50000g of water, 3000g of rice bran, 500g of yeast, 500g of phosphate fertilizer, 6000g of nitrogen fertilizer, 3000g of seaweed, 500g of crushed bone meal, and 500g of fis...

Embodiment 2

[0049] One aspect of this embodiment provides a rooting medium for Photinia fragrans tissue culture seedlings. The rooting medium for Photinia fragrans tissue culture seedlings takes MS medium as the basic medium, and adds 10 g / L of sucrose and 2.55 g of activated carbon to the MS medium. g / L, organic fertilizer component 1.5g / L, bactericidal powder 2.6ml / L, inorganic salt composition 0.5g / L, heat with slow fire, and keep stirring to dissolve the above components, add 1mol / L hydrochloric acid to adjust pH value, make the pH value of the rooting medium between 5.4-6.1, then add 3-6g of agar, stir and put it into a pressure sterilizer, and sterilize at 121°C for 20-30min.

[0050] The organic fertilizer component formula is as follows: 3000g of wheat bran, 500g of lactic acid bacteria, 500g of corn steep liquor, 50000g of water, 3000g of rice bran, 500g of yeast, 500g of phosphate fertilizer, 6000g of nitrogen fertilizer, 3000g of seaweed, 500g of crushed bone meal, and 500g of f...

Embodiment 3

[0059] One aspect of this embodiment provides a rooting medium for Photinia fragrans tissue-cultured seedlings. The rooting medium for Photinia fragrans tissue-cultured seedlings takes MS medium as the basic medium, and adds 19 g / L of sucrose and 1.7 g of activated carbon to the MS medium. g / L, organic fertilizer component 1.7g / L, bactericidal powder 1.9ml / L, inorganic salt composition 1.1g / L, heat with slow fire, and keep stirring to dissolve the above components, add 1mol / L hydrochloric acid to adjust PH value, so that the pH value of the rooting medium is between 5.4-6.0, then add 3-4g of agar, stir and put it into a pressure sterilizer, and sterilize it at 105-121°C for 20-25min .

[0060] The organic fertilizer component formula is as follows: 3000g of wheat bran, 500g of lactic acid bacteria, 500g of corn steep liquor, 50000g of water, 3000g of rice bran, 500g of yeast, 500g of phosphate fertilizer, 6000g of nitrogen fertilizer, 3000g of seaweed, 500g of crushed bone mea...

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Abstract

The invention belongs to the technical field of plant tissue culture rooting media and provides a photinia serrulata tissue culture seedling rooting medium and a culture method. According to the photinia serrulata tissue culture seedling rooting medium, an MS medium serves as a minimal medium, 10-19 g / L saccharose, 1.5-2.55 g / L activated carbon, 1.5-1.7 g / L organic fertilizer components, 1.5-2.6 ml / L sterilization powder and 0.5-1.8 g / L inorganic salt composition are added into the MS medium, wherein the pH value of the rooting medium ranges from 5.4 to 6.1. Based on the MS medium, balanced and reasonable nutritional requirements are provided for photinia serrulata tissue culture rooting seedlings by adjusting the varieties and concentrations of macroelements, optimizing hormone regulation and control and the like, and the seedlings are promoted to grow rapidly, efficiently and strongly.

Description

technical field [0001] The invention belongs to the technical field of rooting medium for plant tissue culture, and in particular relates to a rooting medium for tissue culture seedlings of Photinia fragrans and a cultivation method. Background technique [0002] Photinia frondosa is a hybrid species of Photinia in the family Rosaceae. It is an evergreen small tree or a multi-branched shrub. Because of its bright red leaves and the characteristics of resistance to plastic pruning, it has become an important ornamental tree species with colorful leaves in recent years. It has been widely used in gardens. important applications. Because Photinia fragrans is a hybrid species, large-scale sexual reproduction is greatly restricted, and the quality of seedlings is poor and uneven, so it is difficult to meet the huge demand of the market. [0003] The propagation methods of Photinia fragrans include cutting propagation, tissue culture propagation, grafting propagation, and sowing ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张安育
Owner HEFEI HUAGAI BIOTECH CO LTD
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