Kit for detecting benign or malignant pulmonary nodules and application thereof

A technology for detecting reagents and pulmonary nodules, which is applied in the field of kits for detecting whether pulmonary nodules are benign or malignant, can solve problems such as slow clinical development, and achieve the effect of reducing surgical risks

Inactive Publication Date: 2017-08-11
SHENZHEN HAPLOX BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Early studies have shown circulating tumor cell (CTC) capture assays as potential biomarker

Method used

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  • Kit for detecting benign or malignant pulmonary nodules and application thereof
  • Kit for detecting benign or malignant pulmonary nodules and application thereof
  • Kit for detecting benign or malignant pulmonary nodules and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: the composition of the kit that detects pulmonary nodule as benign or malignant

[0062] Table 1: Components of kits for detecting lung nodules as benign or malignant

[0063] Numbering name volume Specification A Streptavidin Magnetic Beads 5mL 24 times B Adapter 120μL 24 times C PCR primers 2OD 24 times D Pulmonary nodule 358 gene probe 24 μL 24 times E h 2 o

2mL

[0064] The above table 1 defines the composition of the kit for detecting pulmonary nodules as benign or malignant, and some components in the kit are further described below:

[0065] A) The streptavidin magnetic beads in the kit of the present application are used to purify the samples and PCR products by magnetic bead adsorption method treated with streptavidin. It can specifically bind to biotin at the 3' end of the probe to purify and enrich PCR products.

[0066] B) The adapter in the kit of this application is d...

Embodiment 2

[0081] Example 2: Genetic detection of pulmonary nodules in two patients (two samples are samples of benign pulmonary nodules numbered LN201601 and malignant pulmonary nodules of LN201602 respectively)

[0082] The detection method comprises the following steps:

[0083] 1. Collect blood samples from patients and separate them. The details of blood separation are as follows:

[0084] The drawn peripheral blood needs to be stored at 4°C and processed within two hours.

[0085] (1) Centrifuge at 1600g for 10min at 4°C, and divide the supernatant (plasma) into multiple 2.0mL centrifuge tubes after centrifugation. The blood cells in the lower layer are divided into 2mL cryopreservation tubes, which are the required blood cells;

[0086] (2) Centrifuge at 16,000 g for 10 min at 4°C to remove residual cells, and transfer the supernatant into a new 2.0 mL centrifuge tube to obtain the desired plasma. The samples were then stored in a -80°C freezer or dry ice for determination.

...

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Abstract

The invention discloses a kit for detecting benign or malignant pulmonary nodules and an application thereof. The kit comprises the following components: (A) a detection reagent, wherein the detection reagent is magnetic beads treated by streptavidin; (B) a first-type detection component which is an independently designed adapter, wherein the sequence of partial nucleotide is shown in SEQ ID NO:1; (C) a second-type detection component, wherein the detection component is a primer designed according to 358 genes related to the pulmonary nodules, and the sequence of partial primers is shown in SEQ ID NO:2 and SEQ ID NO:3; and (D) a third-type detection component, wherein the third-type detection component is 75642 capture probes designed according to 358 genes related to the pulmonary nodules, and the sequence of partial probes is shown in SEQ ID NO:4 and SEQ ID NO:5. The kit can be used for screening early diseases of lung cancer and discriminating the benign and malignant pulmonary nodules.

Description

technical field [0001] The application relates to the field of gene detection, in particular to a kit for detecting benign or malignant pulmonary nodules and its application. Background technique [0002] Lung cancer is the malignant tumor with the highest morbidity and mortality in the world, with an annual death toll of about 1.4 million, accounting for 18% of all malignant tumor deaths. Professor Peto, a famous British oncologist, predicted that if China does not control smoking and air pollution in time, by 2025, there will be more than 1 million new lung cancer patients every year. With existing detection methods, about 75% of lung cancer patients are diagnosed at an advanced stage, and the 5-year survival rate is about 15.6%. The public lacks screening awareness and lacks scientific methods to identify pulmonary nodules, which keeps the incidence of lung cancer at a high level. Lung cancer is manifested in the form of pulmonary nodules. Therefore, the key to early sc...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6827C12Q1/6886C12Q2600/156C12Q2525/191C12Q2525/301C12Q2531/113C12Q2535/122
Inventor 程方胜刘园园张晓妮温媛
Owner SHENZHEN HAPLOX BIOTECH
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