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Preparation and application of a photoelectrochemical cell sensor for sensitive detection of cancer cells

A cell sensor and photoelectrochemical technology, applied in the field of nanomaterials, can solve the problems of operational complexity, high cost, time-consuming, etc., and achieve the effects of improved sensitivity, good electrical conductivity, and high biocompatibility

Inactive Publication Date: 2019-07-05
UNIV OF JINAN
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Problems solved by technology

Although these analytical methods are able to achieve signal amplification, they are usually operationally complex and time-consuming, labor-intensive, and costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Sensitive photoelectrochemical cell sensor is used for the detection of MCF-7 cell

[0020] (1) First clean the ITO conductive glass, ultrasonically wash it in ethanol, acetone, and secondary water for 15 min, and then spin-coat the seed solution on the surface of the cleaned ITO conductive glass. The seed solution is 40 mM acetic acid zinc solution, then dried at 150 °C for 10 min, and repeated the "spin coating-drying" process 6 times, then inserted the ITO conductive glass into a 25 mL autoclave containing 10 mL growth solution, and made its conductive side face down, as described The growth solution of the method is a mixture of hexamethylenetetramine and zinc nitrate, the concentration of which is 25 mM and the molar ratio is 1:1. It is heated at 90 °C for 6 h, and after it is naturally cooled to room temperature, it is washed with secondary water. And dry at 150 ℃ for 30 min;

[0021] (2) Continue to repeat the "spin coating-drying" process in step ...

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PUM

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Abstract

The invention discloses preparation and application research of a photoelectrochemical cell sensor used for sensitive detection of cancer cells. Three-dimensional multi-dendritic zinc oxide nanorods grow on indium tin oxide conductive glass via an in-situ growing method and are used for loading of a great number of low-toxicity graphene quantum dots and a hairpin DNA probe with one end labeled by silver selenide quantum dots; and the loaded quantum dots and zinc oxide can form good dual sensitization structures so as to realize preliminary signal amplification. Further signal amplification is realized via sensitization inhibiting effect caused by changes in hairpin DNA conformation induced by specific recognition of cancer cells and via the steric-hinerance effect of cells, so sensitive detection of cancer cells is realized. The photoelectrochemical cell sensor constructed in the invention has excellent analysis performance and shows good application prospects in the aspect of clinical diagnosis and treatment of cancers.

Description

technical field [0001] The invention relates to the fields of nanomaterial technology, cancer cell analysis and detection technology and photoelectrochemical signal detection technology, and more specifically relates to the preparation and application of a photoelectrochemical cell sensor for sensitive detection of cancer cells. Background technique [0002] Cancer has always been a major health killer threatening human survival, and the sensitive detection of cancer cells is of great significance to the diagnosis and treatment of cancer. Usually, the content of cancer cells in serum is very low, so the search for highly sensitive cancer cell detection methods has attracted widespread attention. [0003] The photoelectrochemical analysis method is a newly developed detection technology in recent years. Its excitation light source and detection signal are two completely different forms, which can effectively reduce the interference of background signals, thereby greatly impro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/63G01N27/26G01N27/30
CPCG01N21/63G01N27/26G01N27/30
Inventor 杨红梅张彦李丽徐金梦于京华葛慎光颜梅
Owner UNIV OF JINAN
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