Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for isolation and induction of tumor infiltrating T lymphocytes

A technology of tumor infiltration and lymphocytes, applied in the field of biomedicine, can solve the problems of time-consuming, difficult separation and culture, etc., and achieve the effect of strong cell proliferation ability, strong tumor cell killing activity, and high purity

Active Publication Date: 2019-11-01
北京奥康华医学检验所有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the fact that this classic method of isolating and identifying TIL cell tumor-killing activity needs to isolate and induce and culture primary tumor cells as target cells from patient tumor tissue, and co-culture (24h) TIL cells and target cells in vitro, it is time-consuming. It is also quite difficult to isolate and culture primary tumor cells and tumor-infiltrating CD8+ T lymphocytes from tumor tissues. Therefore, it is urgent to establish an efficient and easy method to separate and identify tumor-infiltrating killer T lymphocytes in order to Immunotherapy for Tumors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for isolation and induction of tumor infiltrating T lymphocytes
  • A method for isolation and induction of tumor infiltrating T lymphocytes
  • A method for isolation and induction of tumor infiltrating T lymphocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Separation of autologous tumor cells and TIL

[0035] 1. Routine tumor tissue TIL cell culture

[0036] (1) Place fresh tumor tissue (example is fresh liver cancer tissue taken from surgical resection, with informed consent of the patient) successively placed in a medium containing penicillin-streptomycin double antibody (penicillin working concentration is 100U / ml; streptomycin working concentration is 100U / ml; streptomycin working concentration is Soak in 0.1mg / ml PSB for 3 to 5 minutes, wipe dry with a sterile cotton swab, and then use double antibody containing penicillin-streptomycin (working concentration of penicillin is 100U / ml; working concentration of streptomycin is 0.1mg / ml ) with PBS for 3 to 5 times to wash away the blood and mucus as far as possible. Place it in a sterile petri dish, remove necrosis and adipose tissue with ophthalmic scissors, and keep the tumor tissue.

[0037] (2) Cut the tumor tissue to about 5mm in a sterile petri dish 3 ...

Embodiment 2

[0048] Example 2, Identification of Tumor Tissue-Specific TIL Cells

[0049] 1. TIL cell expansion multiple detection

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a separated induction method for tumor infiltration T lymphocyte. The method for culturing the tumor infiltration T lymphocyte through separated induction from solid tumor comprises the following steps: The method comprises the following steps: 1) adding tumor tissue masses with size of 2-3 mm<3> in a culture aperture containing a separated induction nutrient solution for culture; and the separated induction nutrient is a RPMI-1640 complete medium containing IL-7 and IL-15; 2) performing half medium change every 3-4 days; and 3) after 2-3 weeks, removing the tumor tissue masses, and culturing the cells which are the tumor infiltration T lymphocyte. The TIL obtained by separation has the advantages of high purity, and stronger tumor cells killing activity.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for separating and inducing tumor infiltrating T lymphocytes. Background technique [0002] Malignant tumors have become the leading cause of death in developed countries and the secondary cause of death in developing countries. For the treatment of tumors, the current main treatment methods include traditional radiation therapy, surgical resection of tumors, drug therapy and emerging tumor biological therapy. Because surgery, radiotherapy and chemotherapy will bring huge toxic and side effects to patients, biological therapy has become the fourth important tumor treatment mode by virtue of its significant curative effect. Tumor biological therapy mainly includes: adoptive cell therapy, cytokine therapy, tumor vaccine, targeted molecular therapy, etc. At present, there are many studies on adoptive cell therapy, including tumor infiltrating T lymphocytes (TIL), and among these T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2501/2307C12N2501/2315
Inventor 卢戌王燕飞梁孟儒刘静维刘雪松王跃黄彩庭李京坡吴璇
Owner 北京奥康华医学检验所有限公司