Method for nondestructive testing of polyphenol content in waxberries based on near-infrared hyperspectral technique
A near-infrared hyperspectral and phenol content technology is applied in the field of non-destructive detection of polyphenol content in red bayberry based on near-infrared hyperspectral technology. It can solve the problems of destroying the detection sample and the difficulty of realizing rapid non-destructive detection of large sample sizes, and achieves an increase in detection rate. , Reduce the amount of data calculation and shorten the scanning time
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[0029] Example one
[0030] 1) Establishment of sample spectrum:
[0031] Collect fresh bayberry samples (400 pieces) of different varieties and randomly allocate them, establish a calibration sample set (200 pieces) and a test sample set (200 pieces); use a hyperspectral imaging system to perform spectral scanning on the samples in the calibration and test sample sets. The sample distance is 10cm, the exposure time is 0.5s, the sample moving speed is 5mm / s, and the hyperspectral image of 1000~1600nm near infrared band is collected, and the spectrum of the calibration and inspection sample set is obtained;
[0032] 2) Determination of sample polyphenol content:
[0033] The content of polyphenols in bayberry samples was determined by the Folin-phenol method. The samples were extracted with 90% ethanol, and an appropriate amount of the extract was taken to react with Folin, and the detection was carried out at a wavelength of 760nm, and gallic acid was used as a standard for quantifica...
Example Embodiment
[0042] Example two
[0043] A method for non-destructive determination of polyphenol content in bayberry based on near-infrared hyperspectroscopy, characterized in that the steps of the method are as follows:
[0044] 1) Establishment of sample spectrum:
[0045] Collect fresh bayberry samples (600 pieces) of different varieties and randomly allocate them, establish a calibration sample set (400 pieces) and a test sample set (200 pieces); use the hyperspectral imaging system to perform spectral scanning on the samples in the calibration and test sample sets, and the lens The distance to the sample is 40cm, the exposure time is 3s, the sample moving speed is 15mm / s, and the hyperspectral image in the near infrared band of 900~1700nm is collected to obtain the corrected and inspected sample set spectrum;
[0046] 2) Determination of sample polyphenol content:
[0047] The content of polyphenols in bayberry samples was determined by the Folin-phenol method. The samples were extracted with...
Example Embodiment
[0056] Example three
[0057] A method for non-destructive determination of polyphenol content in bayberry based on near-infrared hyperspectroscopy, characterized in that the steps of the method are as follows:
[0058] 1) Establishment of sample spectrum:
[0059] Collect fresh bayberry samples (300 pieces) of different varieties and randomly distribute them, establish a calibration sample set (200 pieces) and a test sample set (100 pieces); use a hyperspectral imaging system to perform spectral scanning on the samples in the calibration and test sample sets, where the lens and The sample distance is 20cm, the exposure time is 2s, the sample moving speed is 10mm / s, and the hyperspectral image in the near infrared band of 900~1700nm is collected to obtain the corrected and inspected sample set spectrum;
[0060] 2) Determination of sample polyphenol content:
[0061] The content of polyphenols in bayberry samples was determined by the Folin-Phenol method. The samples were extracted wit...
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