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Ultrasonically extracted eumenol angelica polysaccharide and preparation method and application thereof

A technology of polysaccharides of Angelica dahurica and Angelica dahurica, which is applied in the field of ultrasonically extracted polysaccharides of Angelica dahurica and its preparation. It can solve the problems that the research on the structure and activity of polysaccharides of Angelica dahurica has not yet been carried out, and the research on polysaccharides of angelica dahurica is not deep enough, so as to achieve good whitening activity and increase the sugar content. Effect

Inactive Publication Date: 2017-08-22
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on polysaccharides of Angelica dahurica is not deep enough
Only a few scholars have analyzed the extraction of polysaccharides from Angelica dahurica, anti-oxidation and enhancing the immune function of animal skin tissue, and no research on the structure and activity of polysaccharides from Angelica dahurica extracted by ultrasonic method has been carried out.

Method used

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  • Ultrasonically extracted eumenol angelica polysaccharide and preparation method and application thereof
  • Ultrasonically extracted eumenol angelica polysaccharide and preparation method and application thereof
  • Ultrasonically extracted eumenol angelica polysaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of Polysaccharides from Radix Angelica dahurica

[0045] The equipment used for ultrasonic extraction in this example is SB25-12DTD ultrasonic cleaning machine (Ningbo Xinzhi Biotechnology Co., Ltd.).

[0046] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0047] A: degreasing and decolorization

[0048] Take the root of Baizhi root, wash it, dry it at 45°C, and crush it through a 40-mesh sieve. Weigh the powder sample of Angelica dahurica, add petroleum ether to reflux and degrease for 4 hours, dry at 45°C, then add 80% ethanol and extract at 80°C for 4 hours to remove part of the pigment and oligosaccharides, evaporate the ethanol and crush it, dry at 45°C for later use . The consumption of sherwood oil is calculated as 3mL by every gram of Chuan Baizhi powder, and the consumption of 80% ethanol is calculated as 2mL per gram of Chuan Baizhi powder.

[0049] B: extract

[0050] Weigh 100 g of the powder ...

Embodiment 2

[0064] Preparation of Polysaccharides from Radix Angelica dahurica

[0065] The equipment used for ultrasonic extraction in this example is SB25-12DTD ultrasonic cleaning machine (Ningbo Xinzhi Biotechnology Co., Ltd.).

[0066] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0067] Step A: Compared with Step A of Example 1, the drying temperature is 50° C., the amount of petroleum ether is 2 mL per gram of Baizhi powder, and the rest of the conditions are the same.

[0068] Step B: Weigh 100 g of the defatted and depigmented powder, add 2 times the amount of distilled water for ultrasonic extraction for 2 hours, centrifuge at 4000 r / min for 5 minutes to collect the supernatant, combine the extracts and concentrate to obtain a concentrate. Step C to step G are the same as in Example 1, to obtain.

Embodiment 3

[0070] Preparation of Polysaccharides from Radix Angelica dahurica

[0071] The equipment used for ultrasonic extraction in this example is SB25-12DTD ultrasonic cleaning machine (Ningbo Xinzhi Biotechnology Co., Ltd.).

[0072] The preparation method of Radix Angelica dahurica polysaccharide comprises the following steps:

[0073] Step A: Compared with Step A of Example 1, the drying temperature is 40°C, the amount of petroleum ether is 4mL per gram of Baizhi powder, and the rest of the conditions are the same.

[0074] Step B: Weigh 100 g of the degreased and depigmented powder, add 6 times the amount of distilled water for ultrasonic extraction 4 times, each time for 0.5 h, centrifuge at 4000 r / min for 5 min to collect the supernatant, combine the extracts and concentrate to obtain a concentrate. Step C to step G are the same as in Example 1, to obtain.

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Abstract

The invention discloses ultrasonically extracted eumenol angelica polysaccharide. The eumenol angelica polysaccharide has alpha-configuration and beta-configuration polysaccharide link modes at the same time, and the apparent molecular weight of the eumenol angelica polysaccharide is 170.07kDa; the eumenol angelica polysaccharide is prepared by the following steps: performing ultrasonic extraction, alcohol precipitation and impurity removal and alcohol precipitation and desalination on an eumenol angelica medicinal material; then performing column chromatographic separation through a DEAE-agarose gel FF column and an agarose gel 6FF gel column. The obtained eumenol angelica polysaccharide has inhibiting action on activity of tyrosinase, and shows a whitening effect nearly equivalent to that of reference kojic acid. Along with increase of concentration, the tyrosinase inhibiting rate is gradually increased, showing an obvious dose-effect relationship. Through adopting an ultrasonic method to extract eumenol angelica polysaccharide, the molecular weight of the obtained eumenol angelica polysaccharide is great, and the activity of the eumenol angelica polysaccharide is not affected.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to ultrasonically extracted polysaccharides from Radix Angelica dahurica and its preparation method and application. Background technique [0002] The medicinal material Baizhi is the dried root of Angelica dahurica (Fisch.exHoffm) Benth.etHook.f. or Hangbaizhi Angelica dahurica (Fisch.exHoffm) Benth.et Hook.f.var.formosana (Boiss) Shan et Yuan . Angelica dahurica is pungent in taste, warm in nature, and returns to the stomach, large intestine, and lung meridians. It has the effects of relieving exterior cold, expelling wind and relieving pain, dredging nasal orifices, drying dampness and stopping belt, reducing swelling and discharging pus, and is used for cold headache, brow bone pain, nasal congestion, runny nose, nasal congestion, nasal sinusitis, toothache, belt Next, the sores are swollen and painful. [0003] Angelica dahurica is one of the 87 kinds of Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A61P17/00
CPCC08B37/0003
Inventor 吴卫唐小琴侯凯王庆李丹丹苏天骄魏威
Owner SICHUAN AGRI UNIV