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Kit for rapid detection of HPV genotypes with reverse dot blotting method

A genotype and kit technology, which is applied in the field of kits for rapid detection of HPV genotypes using reverse dot hybridization method, can solve the problems of inability to perform specific typing, few genotypes, and long detection time.

Inactive Publication Date: 2017-08-25
GUANGZHOU HEAS BIOTECH CO LTD
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Problems solved by technology

[0006] The purpose of the present invention is to provide a kit for quickly detecting HPV genotypes using the reverse dot hybridization method, which solves the problems in the prior art that the detection takes a long time, cannot be specifically typed, and the number of genotypes is so small that it cannot cover as many HPV genotypes as possible. Common HPV infection types and other issues among Chinese people

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  • Kit for rapid detection of HPV genotypes with reverse dot blotting method
  • Kit for rapid detection of HPV genotypes with reverse dot blotting method
  • Kit for rapid detection of HPV genotypes with reverse dot blotting method

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Embodiment Construction

[0082] Below in conjunction with embodiment, further illustrate the present invention, but not limit the present invention.

[0083] The invention relates to a kit for rapidly detecting HPV genotypes by reverse dot hybridization method, comprising HPV typing PCR reagent and reverse dot hybridization reagent.

[0084] The composition of HPV typing PCR reaction solution solvent is as follows:

[0085] Tris-HCl 96mM

[0086] KCl 80mM

[0087] (NH 4 ) 2 SO 4 16mM

[0088] Magnesium acetate TAR 5mM

[0089] Taq enzyme 0.12U / μL

[0090] dNTPs 200.4μM

[0091] Glycerol 4%

[0092] The specific primer sequences are as follows:

[0093] serial number Sequence (5'—3') sequence 1 TGCMCAGGGWCATAAYAATG sequence 2 GCTCAGGGTTTAAACAATGG sequence 3 GCCCAGGGCCACAATAATGG sequence 4 CGTCCMARRGGAWACTG sequence 5 GCGACCCAATGCAAATTG sequence 6 CGTCCTAGTGGAAATTGATC sequence 7 CTACCGAATGGAAATTGATC sequence 8 CGCCCTAAGGGAAAC...

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Abstract

The invention relates to a kit for rapid detection of HPV genotypes with a reverse dot blotting method. The kit for detection of 28 HPV genotypes which cover 18 HPV high-risk types and 10 HPV low-risk types in total is built. The kit comprises two parts of PCR reagents for HPV typing and reverse dot blotting reagents, the HPV types of clinical samples can be detected rapidly, accurately and in high throughput, the kit has a prediction effect on HPV clinical diagnosis and cervical cancer prognosis, and the problems that in the prior art, detection is time-consuming, specific typing cannot be carried out, and the genotypes classified are few, so that common HPV infection types of countrymen are not covered as many as possible are solved.

Description

technical field [0001] The invention relates to a HPV detection kit, in particular to a kit for rapidly detecting HPV genotypes by reverse dot hybridization. Background technique [0002] Cervical cancer is one of the most common malignant tumors in women. There are about 130,000 new cases of cervical cancer in my country every year. It is an important cause of cancer-related death in women after breast cancer. In recent years, with the change of lifestyle, the age of onset of cervical cancer patients is getting younger, and the number of cases is on the rise. Nearly 500,000 women worldwide are diagnosed with cervical cancer every year. Cervical cancer is the only cancer whose cause is relatively clear. The main cause is persistent infection or repeated infection of high-risk human papillomavirus (HPV). As a result, HPV is a virus that specifically infects human epidermis and mucosal squamous epithelium, and its transmission route is mainly through sexual behavior. HPV inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/708C12Q2600/112C12Q2521/101
Inventor 刘淑园陈华云肖湘文丁渭赵丽
Owner GUANGZHOU HEAS BIOTECH CO LTD
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