Method for cultivating strong seedlings for vaccinium uliginosum tissue culture and rapid propagation based on color mixing LED light source

Abstract

The invention belongs to the plant tissue culture and rapid propagation field, and mainly relates to a method for cultivating strong seedlings for vaccinium uliginosum tissue culture and rapid propagation based on a color mixing LED light source. According to the method, red and blue color mixing LED light sources which are in a certain proportion are used for conducting strong seedling treatment respectively in a vaccinium uliginosum seedling multiplication phase and a rooting phase. By adopting the method, the propagation coefficient of vaccinium uliginosum is improved; the rooting cycle is shortened; the growth of stems, leaves and root systems is accelerated; vaccinium uliginosum tissue culture seedlings grow thick and strong; leaf areas are enlarged; and the transplanted survival rate is improved. According to the vaccinium uliginosum tissue culture seedlings cultivated by the method, the content of soluble sugar and anthocyanin in the leaves is obviously improved; the anti-adversity ability of the seedlings can be effectively improved; and the strong seedling transplantation and the subsequent adaptive capacity of the seedlings are improved by a large margin. Compared with used traditional white fluorescent lamps, the red and blue color mixing LED light sources save electric energy effectively; the cost is reduced while the quality of the vaccinium uliginosum tissue culture seedlings is comprehensively improved; and an efficient and economical method for cultivating the strong seedlings for the vaccinium uliginosum tissue culture and rapid propagation is provided.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a method for tissue culture, rapid propagation and strong seedlings of Vaccinium cranberry based on a mixed-color LED light source. Background technique: [0002] Vaccinium uligiosum Linn. is a deciduous shrub of the Rhododendronaceae Vaccinium genus. It is extremely cold-resistant. It is concentrated in the Greater and Lesser Khingan Mountains and Changbai Mountains in China. It is one of the wild berry resources with great development and utilization value. It is commonly known as wild blueberry. . Its fruit is blue-purple, rich in flavonoids, polyphenols, anthocyanins, proanthocyanidins and other active substances. It has significant effects on preventing cancer, cardiovascular, anti-aging, improving eyesight, and improving immunity. It is recognized by the International Food and Agriculture Organization Listed as one of the five major heal...

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for fast-growing seedlings of Vaccinium vaccinium tissue culture based on color-mixing LED light source, which can effectively solve the problems of thin, weak, poor adaptability, and low survival rate of transplanting Vaccinium vaccinia. It can reduce the cost of tissue culture production of lingonberry

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