Method for improving proliferation rate of tissue culture seedlings of Hosta plantaginea

Abstract

The invention discloses a method for improving proliferation rate of tissue culture seedlings of Hosta plantaginea, the method is as follows: a succeeding preservation test-tube plantlets are inoculated into a culture medium, a culture bottle has a volume of 100ml, and is placed in light with the quality of 70% of red light + 30% blue light and with the light intensity of 30-50 mu mol.m<-2>.s<-1>, and the distance between a light source and a bottle mouth is 20cm. The light quality and the light intensity most suitable for proliferation of the tissue culture seedlings of the Hosta plantaginea, especially ''fine blanket'' Hosta plantaginea can be determined by the method, and the method is used in the research field of the tissue culture seedlings of the Hosta plantaginea to reduce operating cost, reduce energy consumption, reduce subculture and reduce the mutation rate, and facilitates in vitro conservation of germplasm resources of the Hosta plantaginea, and maximizes the economic benefits of Hosta plants.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for increasing the proliferation rate of hosta tissue culture seedlings. Background technique [0002] Hosta is a perennial herbaceous flower of Liliaceae, mainly distributed in temperate and subtropical regions of East Asia. The diversity of leaf shape, size, color and texture is the main ornamental value of hosta plants. Many varieties of yellow, blue-gray, green and double-colored four major leaf lines have been cultivated through various ways. By the end of the last century, hosta has become the world's best-selling herbal flower, with high economic value and large market demand. [0003] Due to the fast propagation speed, the group-cultivated seedlings are not restricted by external climate, geographical and time conditions. At present, it has become an important means of plant genetic breeding, germplasm resource protection and virus-free and rapid...

AI Technical Summary

Problems solved by technology

Traditional tissue culture lighting systems mostly use fluorescent lamps, which have low light efficiency and more light quality that plants cannot use; the heat generation is large, which increases the power consumption of air conditioning and cooling; plants cannot be irradiated at close range, and the culture vessels will condense due to the heat dissipation of lamps and other shortcomings

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