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Tumor tissue in-vitro preservation liquid and preparation method thereof

A tumor tissue and preservation solution technology, applied in the biological field, can solve problems such as functional limitations, limited activity of tumor cells, and low success rate of tumor tissue transplantation, and achieve the effects of prolonging the storage time, increasing the success rate, and promoting growth

Inactive Publication Date: 2017-08-29
南通普惠精准医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The inventors of the present application found in the long-term research and development process that the PBS buffer solution is simple in composition, but its function is limited, and it cannot well maintain the biological activity of tumor tissue in vitro; the cell culture medium is mainly used to maintain the growth and proliferation of cells, not as a tumor The best choice for tissue preservation solution
When the above two are used as tumor tissue preservation solutions in vitro, the activity of retaining tumor cells is limited, and the longer the storage time in vitro, the worse the activity of tumor cells, resulting in a low success rate of tumor tissue transplantation

Method used

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  • Tumor tissue in-vitro preservation liquid and preparation method thereof
  • Tumor tissue in-vitro preservation liquid and preparation method thereof
  • Tumor tissue in-vitro preservation liquid and preparation method thereof

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preparation example Construction

[0029] See figure 1 , figure 1 It is a schematic flow chart of an embodiment of the preparation method of the tumor tissue in vitro preservation solution of the present invention, and the method includes:

[0030] Step S101: Add antibiotics to the basic medium to obtain liquid A;

[0031] Step S102: Dissolve taurine in a natural medium, and then add polyethylene glycol octyl phenyl ether to obtain liquid B;

[0032] Step S103: adding liquid B to liquid A and mixing to obtain an in vitro preservation solution of tumor tissue.

[0033] It should be noted that the various components used in the preparation of the tumor tissue in vitro preservation solution in this embodiment are the same as those in the first and second embodiments of the above-mentioned tumor tissue in vitro preservation solution of the present invention. For a detailed description of the relevant content, please Please refer to the above-mentioned embodiment, which will not be repeated here.

[0034] In an application s...

Embodiment 1

[0041] Divide the same volume of PBS buffer solution, cell culture solution and the tumor tissue in vitro preservation solution 1, 2, and 3 of the present invention into 5 groups as the preservation solution to preserve the tumor tissue. Among them, the preserved tumor tissue is of equal weight. The above 5 groups of tumor tissue transplantation experiments were completed within 2 hours. Each group was transplanted 5 6-week-old non-obese diabetes / severe combined immunodeficiency (Non ObeseDiabetes-Server Combined Immune-deficiency, NOD-SCID) mice subcutaneously. One month later, the tumorigenesis status of the tumor tissue was observed, and the success rate of colon cancer transplantation in the three groups was compared, as shown in Table 2. Compared with the other two groups, P <0.05.

[0042] It needs to be pointed out that, except for the above-mentioned differences, other conditions are the same.

[0043] Table 2 The success rate of colon cancer transplantation with each pre...

Embodiment 2

[0047] Divide the same volume of PBS buffer solution, cell culture solution and the tumor tissue in vitro preservation solution 1, 2, and 3 of the present invention into 5 groups as the preservation solution to preserve the tumor tissue. Among them, the preserved tumor tissue is of equal weight. The above 5 groups of tumor tissue transplantation experiments were completed within 6 hours. Each group was transplanted with 5 6-week-old NOD-SCID mice subcutaneously. One month later, the tumorigenesis status of tumor tissues was observed, and the success rate of colon cancer transplantation in the three groups Make a comparison, as shown in Table 3. Compared with the other two groups, P <0.05.

[0048] It needs to be pointed out that, except for the above-mentioned differences, other conditions are the same.

[0049] Table 3 The success rate of colon cancer transplantation with each preservation solution

[0050]

PBS buffer

Cell culture medium

Preservation solution 1

Preservatio...

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Abstract

The invention discloses tumor tissue in-vitro preservation liquid and a preparation method thereof. The method comprises the following steps: adding antibiotics into a basic culture medium to obtain a solution A; dissolving taurine into a natural culture medium and adding polyethylene glycol octylphenol ether to obtain a solution B; adding the solution B into the solution A and uniformly mixing to obtain the tumor tissue in-vitro preservation liquid. The in-vitro preservation liquid prepared by the method can be used for keeping the bioactivity of tumor tissues in an in-vitro preservation process, prolonging the in-vitro preservation time of the tumor tissues and improving the success rate of tumor transplantation.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a tumor tissue in vitro preservation solution and a preparation method thereof. Background technique [0002] The Patient-Derived Xenograft (PDX) model of human-derived tumor is a model in which tumors from cancer patients are directly transplanted into mice to grow tumors. Because it is highly consistent with the real clinical level and can accurately reflect the pathological characteristics and drug response of the tumor patients from which it originated, it has become the preferred model for the development of anticancer drugs by major pharmaceutical companies. The construction of the PDX model requires fresh human tumor tissue samples. Therefore, in order to ensure the success rate of transplantation, during the tumor tissue transplantation experiment, a preservation solution is needed to maintain the biological activity of the tumor tissue during the transplantation process to improv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 王国华王金星宋炜
Owner 南通普惠精准医疗科技有限公司
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