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Method for extracting chlorophylls by utilizing biological enzymes

A technology of chlorophyll and biological enzymes, applied in the field of agricultural biology, can solve problems such as environmental pollution, waste of biomass energy, immaturity, etc., and achieve the effects of low energy consumption, wide sources, and easy operation

Inactive Publication Date: 2017-08-29
李茂兰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the comprehensive development and utilization technology of rice straw and other biomass is still immature, and most of rice straw and other biomass are incinerated, which seriously pollutes the environment and is also a huge waste of biomass energy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting chlorophyll using biological enzymes, comprising the following steps:

[0029] (1) Crushing: Wash the fresh rice stalks, dry them, and crush them into 1-3 mm particles;

[0030] (2) Hydrolysis: The pulverized particles were subjected to acid hydrolysis under stirring, the hydrolysis pressure was 0.2MPa, the temperature was 100°C, pH 2.0, the stirring speed was 100 rpm, and the hydrolysis time was 3 h;

[0031] (3) Filtration: After the hydrolysis is completed, filter to obtain the hydrolyzate;

[0032] (4) Enzyme addition: adjust the temperature of the hydrolyzate to 50°C, add 2000 U / g ligninase 0.5 g / L and 4000 U / g cellulase 3 g / L, and enzymatically hydrolyze for 150 min;

[0033] (5) Enzyme inactivation: heat the hydrolyzate to 70°C for 10 min to inactivate ligninase and cellulase;

[0034] (6) Cooling: Cool the hydrolyzate to 55°C, add 10000 U / g amylase 0.5 g / L, 200000 U / g papain 0.1 g / L, and hydrolyze for 5 h;

[0035] (7) Separation: Cent...

Embodiment 2

[0038] (1) Crushing: Wash the fresh rice stalks, dry them, and crush them into 1-3 mm particles;

[0039] (2) Hydrolysis: The pulverized particles were subjected to acid hydrolysis under stirring, the hydrolysis pressure was 0.2MPa, the temperature was 110°C, pH 2.0, the stirring speed was 100 rpm, and the hydrolysis time was 1 h;

[0040] (3) Filtration: After the hydrolysis is completed, filter to obtain the hydrolyzate;

[0041] (4) Enzyme addition: adjust the temperature of the hydrolyzate to 55°C, add 2000 U / g ligninase 1.0 g / L and 4000 U / g cellulase 5 g / L, and enzymatically hydrolyze for 120 min;

[0042] (5) Enzyme inactivation: heat the hydrolyzate to 75°C for 5 minutes to inactivate ligninase and cellulase;

[0043] (6) Cooling: Cool the hydrolyzate to 55°C, add 10000 U / g amylase 0.2 g / L, 200000 U / g papain 0.6 g / L, and enzymatically hydrolyze for 3 h;

[0044] (7) Separation: Centrifuge at a speed of 3000 rpm to separate and obtain chlorophyll;

[0045] (8) Drying:...

Embodiment 3

[0047] (1) Crushing: Wash the fresh rice stalks, dry them, and crush them into 1-3 mm particles;

[0048] (2) Hydrolysis: acid hydrolyze the pulverized particles under stirring, hydrolysis pressure 0.3MPa, temperature 100°C, pH 5.0, stirring speed 50, hydrolysis time 3 h;

[0049] (3) Filtration: After the hydrolysis is completed, filter to obtain the hydrolyzate;

[0050] (4) Enzyme addition: adjust the temperature of the hydrolyzate to 50°C, add 2000 U / g ligninase 1.0 g / L and 4000 U / g cellulase 2 g / L, and enzymatically hydrolyze for 180 min;

[0051] (5) Enzyme inactivation: heat the hydrolyzate to 70°C for 5 minutes to inactivate ligninase and cellulase;

[0052] (6) Cooling: Cool the hydrolyzate to 50°C, add 10000 U / g amylase 0.5 g / L, 200000 U / g papain 0.1 g / L, and hydrolyze for 8 h;

[0053] (7) Separation: Centrifuge at a speed of 2000 to separate and obtain chlorophyll;

[0054] (8) Drying: Vacuum-dry the chlorophyll for 30 minutes at a temperature of 10°C and a vacu...

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PUM

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Abstract

The invention discloses a method for extracting chlorophylls by utilizing biological enzymes. The method comprises the eight steps of crushing, hydrolyzing, filtering, enzyme-adding, enzyme deactivation, cooling, separating and drying; a chlorophyll product is prepared from rice straws. The method disclosed by the invention simultaneously solves the difficulties of chlorophyll extraction and rice straw treatment and has good ecological benefits, economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a method for extracting chlorophyll by using biological enzymes. Background technique [0002] Chlorophyll is a unique component in plants. Chlorophyll is a very important and even indispensable substance for human beings. Chlorophyll is rich in trace element iron and is a natural hematopoietic raw material. Without chlorophyll, blood cannot be produced continuously. Anemia occurs in the human body. Chlorophyll also contains a large amount of vitamin C and inorganic salts, which are indispensable substances in human life activities, and can maintain the weak alkalinity of body fluids, which is beneficial to health. Chlorophyll also plays an important role in the production and maintenance of enzyme activity. Chlorophyll is also the best natural detoxifier, which can prevent colds, prevent the spread of inflammation, and has the function of pain relief. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22
CPCC07D487/22
Inventor 李茂兰蒋军
Owner 李茂兰