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Enzymatic packing for reactive distillation as well as coating method and application of enzymatic packing

A technology of catalytic packing and reactive distillation, applied in chemical instruments and methods, catalyst activation/preparation, physical/chemical process catalysts, etc., can solve the problem that the activity and stability of free enzymes are easily affected by conditions such as temperature and pH, free enzymes Enzyme can not be recycled, waste economy and other problems, to achieve the effect of obvious economy and novelty, mild conditions, and reduce pressure drop

Inactive Publication Date: 2017-09-01
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If free enzymes are used in reactive distillation, there are several limiting factors: 1. The activity and stability of free enzymes are easily affected by conditions such as temperature and pH; 2. Free enzymes cannot be recycled, resulting in waste and economic losses.

Method used

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  • Enzymatic packing for reactive distillation as well as coating method and application of enzymatic packing
  • Enzymatic packing for reactive distillation as well as coating method and application of enzymatic packing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of solution A: Mix 0.35 g of tetramethoxysilane (TMOS), 1.4 g of methyltrioxymethylsilane, and 1.6 g of methanol, respectively, and stir in an ice-water bath for 3 minutes.

[0031] Preparation of solution B: Take 0.225g of sodium fluoride, 0.075g of polyethylene glycol (400), 0.8g of CALB enzyme solution (protein content is 10.9mg / ml), and 0.55g of deionized water to mix and stir at room temperature 4 minutes.

[0032] Drain solution B into solution A, stir in an ice-water bath for 3 minutes and then move to room temperature.

[0033] The sol was divided into two equal parts, one part was washed three times with phosphate buffer (PH=7.5) and then dried to obtain a gel, as shown in the attached figure 1 shown. The other part was immersed in the sol of CY-500 type filler for 20s, then taken out, placed at room temperature for 20s, then immersed in the sol again, and this step was repeated 12 times until the sol solution solidified into a gel. Dry the gel-c...

Embodiment 2

[0037] Preparation of solution A: Mix 0.4 g of tetramethoxysilane (TMOS), 1.45 g of methyltrioxymethylsilane, and 1.7 g of methanol respectively, and stir in an ice-water bath for 3 minutes.

[0038] Preparation of solution B: get 0.25g sodium fluoride, polyethylene glycol (400) 0.075g, CALB enzyme solution 0.525g (protein content is 10.9mg / ml), deionized water 0.6g to mix respectively, stir at room temperature for 4 minute.

[0039] Drain solution B into solution A, stir in an ice-water bath for 3 minutes and then move to room temperature.

[0040] Immerse the CY-500 type filler in the sol for 20 seconds, take it out, place it at room temperature for 20 seconds, and then immerse it in the sol again. Repeat this step 13 times. Dry the filler at room temperature for 48 hours until the weight remains constant, and then store it at low temperature.

[0041] Test the activity of CALB enzyme contained in the filler surface coating prepared above: the enzyme activity was measured...

Embodiment 3

[0044] Preparation of solution A: Mix 0.9 g of tetramethoxysilane (TMOS), 2.7 g of methyltrioxymethylsilane, and 3.1 g of methanol, respectively, and stir in an ice-water bath for 3 minutes.

[0045] The preparation of solution B: get 0.4g sodium fluoride, polyethylene glycol (400) 0.2g, CALB enzyme solution 1.4g (protein content is 10.9mg / ml), deionized water 1.3g to mix respectively, stir at room temperature for 4 minute.

[0046] Drain solution B into solution A, stir in an ice-water bath for 3 minutes and then move to room temperature.

[0047] Immerse the CY-500 type filler in the sol for 20 seconds, take it out, place it at room temperature for 20 seconds, and then immerse it in the sol again. Repeat this step 10 times. Dry the filler at room temperature for 48 hours until the weight remains constant, and then store it at low temperature.

[0048] Test the activity of CALB enzyme contained in the filler surface coating prepared above: the enzyme activity was measured ...

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Abstract

An enzyme-catalyzed filler for reactive distillation and its coating method and application. The enzyme-catalyzed filler is prepared by the following method, including the following steps: immerse the filler in the sol containing biological enzyme for 20-30 seconds, take it out and dry it for 10-20 seconds; then immerse the filler in the sol again for 20-30 seconds, Take it out again and dry for 10 to 20 seconds; repeat the immersion-drying step 8 to 15 times; finally dry the filler until the weight remains unchanged, that is, obtain the enzyme-catalyzed filler with a biological enzyme coating attached; the composition of the sol is component A and component B; the A component includes an oxygen-containing silane mixture and a solvent; the B component includes a catalyst, a cross-linking agent, an enzyme solution and deionized water. The invention realizes the integration of biological enzyme immobilization technology and reactive distillation technology, and has novelty and economy.

Description

technical field [0001] The present invention relates to the preparation of a kind of biological enzyme coating and the method for coating it on the filler surface, specifically candida antarctica lipase B (candida antarctica lipase B ( CALB) coating belongs to the field of combination of immobilized enzyme technology and reactive distillation. Background technique [0002] As a biocatalyst, enzyme has the advantages of strong catalytic ability, high efficiency, mild reaction conditions, and no pollution to the environment, and has incomparable advantages over conventional chemical catalysts. Candida antarctica lipase B (CALB) is a lipase that has strong catalytic activity for both water-insoluble and water-soluble substances. Enzymes have better catalytic performance. [0003] Enzyme immobilization is to combine the original water-soluble enzyme with a solid non-water-soluble support or be embedded by a carrier by chemical or physical treatment. There are four main types ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/00B01J37/02C12N11/04
CPCB01J37/0217B01J31/003B01J37/0219B01J37/0228B01J37/0236C12N11/04
Inventor 王洪海李旭姜艳军薛璐璐刘文静李春利李浩
Owner HEBEI UNIV OF TECH
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