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Phellinus linteus solid culture as well as preparation method and application thereof

A technology for cultivating phyllobacteria and solids, applied in the field of applied biology, can solve problems such as cumbersome separation and purification procedures, and achieve the effects of low cost, simplified production process, and simple process

Active Publication Date: 2017-09-05
GUANGZHOU GENSOURCE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Conventional cultivation of medicinal fungi generally uses wood chips as a medium to cultivate fruiting bodies, and then extracts the active ingredients from the fruiting bodies. The separation and purification procedures are very cumbersome

Method used

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  • Phellinus linteus solid culture as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of preparation method of Phellinus linteus solid culture of the present embodiment, comprises the following steps:

[0033] 1) Add 50 grams of wheat germ and 1 gram of soybean powder to 100 grams of water, put it in a wide-mouth glass bottle, seal it with gauze, put it in a steam pressure cooker, 1kg / cm 2 , sterilized for 30 minutes to obtain the culture medium;

[0034] 2) inserting Phellinus linteus slant strains into the sterilized culture medium;

[0035] 3) After inoculation, it is placed in a constant temperature incubator and cultured at 25-28°C for 30 days to obtain the product.

Embodiment 2

[0037] A kind of preparation method of Phellinus linteus solid culture of the present embodiment, comprises the following steps:

[0038] 1) Add 40 grams of wheat germ and 2 grams of soybean powder to 100 grams of water, put it in a wide-mouth glass bottle, seal it with gauze, put it in a steam pressure cooker, 1kg / cm 2 , sterilized for 30 minutes to obtain the culture medium;

[0039] 2) inserting Phellinus linteus slant strains into the sterilized culture medium;

[0040] 3) After inoculation, it is placed in a constant temperature incubator and cultured at 25-28°C for 30 days to obtain the product.

Embodiment 3

[0042] A kind of preparation method of Phellinus linteus solid culture of the present embodiment, comprises the following steps:

[0043] 1) Add 30 grams of wheat germ and 3 grams of soybean powder to 100 grams of water, put it in a wide-mouth glass bottle, seal it with gauze, put it in a steam pressure cooker, 1kg / cm 2 , sterilized for 30 minutes to obtain the culture medium;

[0044] 2) inserting Phellinus linteus slant strains into the sterilized culture medium;

[0045] 3) After inoculation, it is placed in a constant temperature incubator and cultured at 25-28°C for 30 days to obtain the product.

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Abstract

The invention discloses a phellinus linteus solid culture as well as a preparation method and application thereof. The phellinus linteus solid culture is obtained by inoculating phellinus linteus into a sterilized culture medium, putting the culture medium into a constant-temperature culture box and culturing at 25 DEG C to 28 DEG C for 10 to 30 days; the culture medium contains 100g / L to 1000g / L of wheat germs and 10g / L to 100g / L of soybean meal. The phellinus linteus solid culture disclosed by the invention adopts edible raw materials; the raw materials are biologically transformed into active substances capable of adjusting blood pressure and blood glucose of human bodies through the phellinus linteus; the phellinus linteus solid culture can be infinitely amplified according to requirements and the problem of sources of key raw materials is solved; the solid culture disclosed by the invention can be directly processed into oral solutions which have functions of lowering the blood pressure and lowering the blood glucose and complicated separation and purification procedures are saved, so that a production process is greatly simplified and the production cost is saved; the phellinus linteus solid culture is easily popularized to people having diseases of hypertension and hyperglycemia with low income.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, more specifically, the invention relates to a solid culture of Phellinus linteus and its preparation method and application. Background technique [0002] The fruiting body growth cycle of wild Phellinus linteus can be as long as 20 to 30 years. It can only be collected in virgin forests. The resources are quite scarce and the price is quite expensive. [0003] According to literature records, the hot water extract of the fruiting body of Phellinus linteus has an inhibitory rate of 96.7% on mouse sarcoma 180, and can be used to treat various tumors, liver diseases, heart disease, stroke hemiplegia, stomach disease, gastric Ulcers, loss of appetite and other diseases, is a rare medicinal fungus. [0004] Phellinus linteus, known as the king of anti-cancer, is actually the same species (Phellinus linteus) with different Chinese names according to the identification of modern molecular biology....

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L31/00A61K36/07A61P9/12A61P3/10C12R1/645
CPCA23L31/00A23V2002/00A61K36/07C12N1/14A23V2200/326A23V2200/328
Inventor 涂桂洪邓惠平
Owner GUANGZHOU GENSOURCE BIO TECH CO LTD
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