Preparation method of all-organ acellular matrix of heart

Abstract

The invention discloses a preparation method of all-organ acellular matrix of a heart. In the method, perfusion with 1% SDS solution and 1% Ttiton is performed, then PBS is used for flushing, liquid is exchanged for 4-5 times every day, and cleaning is totally performed for 72-128h. According to the method, a reasonable all-organ acellular system is designed, an existing heart all-organ acellular method is improved and optimized, and acellular treatment is performed according to different eluting sequences and time proportions by combining with SDS detergent and TritonX100, so that the eluting effect of the perfusate in the heart organ is improved, and a foundation is built for application of the method in elevation of late acellular matrix and reconstruction of tissue engineering. The all-organ acellular matrix of the heart prepared by the method can reserve original second-level and third-level vessel structures, supermicro conformation and extracellular matrix components of the heart under the condition of removing original resident cells of the original tissue.

Description

technical field [0001] The invention relates to an acellular matrix, in particular to a method for preparing the acellular matrix of whole heart organs. Background technique [0002] Cardiovascular disease has become one of the diseases with the highest morbidity and mortality in my country and even the world, seriously threatening human health. Among them, the increase in the prevalence of end-stage heart disease has become an important medical problem in cardiovascular diseases, and heart orthotopic allografting is currently the only effective treatment for it. However, heart transplantation is currently facing many challenges, such as serious shortage of working body and immune rejection after transplantation, which greatly limit the clinical application. [0003] The purpose of the whole organ decellularization mechanism of the heart is to retain more effective components of the extracellular matrix (including collagen, glycosaminoglycans, soluble growth factors, etc.) ...

AI Technical Summary

Problems solved by technology

Using the trypsin method to prepare the acellular matrix, the vascular structure is largely destroyed, which is not conducive to tissue regeneration

Although new progress has been made in the field of decellularization, there is still no method suitable for the decellularization of whole cardiac organs, in order to remove the resident cells in the organ as much as possible, reduce the impact on various extracellular matrix components, and obtain Excellent scaffold material with tissue specificity, three-dimensional ultrastructure, vascular structure and certain biological activity

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