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Traditional Chinese medicine extract composition for treating cervical cancer

The technology of extract and composition is applied in the field of natural traditional Chinese medicine extract composition to prepare and treat cervical cancer drug development field, which can solve the problems of large dosage of single Chinese herbal medicine, difficult to control toxic and side effects, limited anti-cancer effect, etc. The process is simple and easy to operate, easy to put into production, and obtain convenient results

Inactive Publication Date: 2017-09-15
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the deficiencies in the prior art in antineoplastic drugs, to overcome the problems of bone marrow function suppression, immune function reduction and drug resistance caused by traditional chemotherapeutic drugs; Insufficient control, limitation of anticancer effect

Method used

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  • Traditional Chinese medicine extract composition for treating cervical cancer
  • Traditional Chinese medicine extract composition for treating cervical cancer
  • Traditional Chinese medicine extract composition for treating cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Observation and determination of drug toxicity test of Chinese medicine extract composition.

[0020] Experimental materials: (1) Experimental cells: human cervical cancer HeLa cell line: purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, subcultured by conventional methods; (2) Experimental drugs: dried fruit of the Chinese medicine Forsythia, produced in Shanxi; Chinese medicine licorice root, produced in Inner Mongolia , were purchased from the Outpatient Department of Guoyitang, Heilongjiang Association of Traditional Chinese Medicine, and passed the identification of the Chinese Medicine Department of Heilongjiang Food and Drug Inspection and Testing Institute.

[0021] Experimental method: (1) Extraction of the ethanol extract of the traditional Chinese medicine Forsythia was purified according to conventional methods: the specific steps were to take the dried fruit of Forsythia, wash and remove dust, soak in distilled water overni...

Embodiment 2

[0027] Example 2: MTT colorimetric detection of the Chinese medicine extract composition on HeLa cell proliferation inhibition rate and cytotoxicity (IC50) determination.

[0028] 1. Experimental materials: the same as in Example 1.

[0029]2. Experimental method: HeLa cells in good logarithmic growth phase were digested into a cell suspension, and the cell density was adjusted with cell culture medium to make it reach 1.0×105 cells / mL. The cell suspension was added to a 96-well plate at 100 μL / well, and incubated in a CO2 incubator for 24 hours. The drug ratio is 2:1, the final drug concentration is 120 μg / mL, 60 μg / mL, 30 μg / mL, 15 μg / mL, 7.5 μg / mL, 3.75 μg / mL, 100 μL per well. And set up forsythia ethanol extract group, licorice acetone extract group, cisplatin (DDP) drug control group, vehicle negative control group (the cell growth will not be affected when the alcohol concentration is lower than 0.5%) and blank control group (only culture solution added) , without cell...

Embodiment 3

[0033] Example 3: The application of flow cytometer AnnexinV-PI double staining method to detect the apoptosis of human cervical cancer HeLa cells induced by the Chinese medicine extract composition.

[0034] Experimental material: with embodiment 1.

[0035] Experimental method: HeLa cells in good logarithmic growth phase were inoculated into 50mL culture flasks, incubated in a 37°C, 5% CO2 incubator, and the number of cells was adjusted to 1×106 cells / mL. Dilute the final concentration of the Chinese medicine extract composition (2:1) to 120 μg / mL, 60 μg / mL, 30 μg / mL, 15 μg / mL, 7.5 μg / mL, and 3.75 μg / mL in a 50 mL culture bottle, and set up a continuous Alice ethanol extract control group, licorice acetone extract control group, vehicle negative control group. Incubate for 24 hours in a 37°C, 5% CO2 incubator. Centrifuge at 4°C and 1000r / min for 10 min, discard the supernatant and collect the cells, repeat the above conditions and wash twice with PBS. The cells were resus...

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Abstract

The invention provides a traditional Chinese medicine extract composition for treating cervical cancer. The traditional Chinese medicine extract composition comprises fructus forsythia and radix glycyrrhizae. The traditional Chinese medicine extract composition is a natural pharmaceutical composition creatively prepared by fructus forsythia ethanol extract and radix glycyrrhizae acetone extract according to the proportion of (1-4):1, and the optimal proportion obtained after repeated experiments is 2:1. Experiments prove that the traditional Chinese medicine extract composition has a proliferation inhibiting effect and an apoptosis-inducing effect on cervical cancer HeLa cells and has an extremely good antitumor effect, and the two traditional Chinese medicine extracts are combined to achieve a synergic effect, reduce drug use amount and lower toxic and side effects. The invention further provides application of the traditional Chinese medicine extract composition in the preparation of drugs for treating the cervical cancer. By the traditional Chinese medicine extract composition, an experimental foundation and scientific basis is provided for the application of traditional Chinese medicine compatibility to clinical antitumor drug researches.

Description

technical field [0001] The invention belongs to the technical field of medicine, and the Chinese medicine extract composition is composed of two traditional Chinese medicines, forsythia and licorice. In particular, it relates to a natural Chinese medicine extract composition composed of forsythia ethanol extract and licorice acetone extract, and the composition of the Chinese medicine extract is used for the preparation and development of drugs for treating cervical cancer. Background technique [0002] Malignant tumors are the number one cause of human death due to disease. Cervical cancer is one of the most common malignant tumors in gynecology. Its incidence rate is lower than that of breast cancer, and its mortality rate is lower than that of ovarian cancer, which ranks first in gynecology. [0003] According to the records in ancient books and prescriptions, breakthroughs have been made in extracting effective components from Chinese herbal medicine plants and applying...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/634A61P15/00A61P35/00
CPCA61K36/634A61K36/484A61K2300/00
Inventor 王吉锡孟丹国立东姜国华
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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