Crop growth regulator based on inonotus obliquus, and preparation method and application thereof

Technology of a growth regulator, Inonotus obliquus, applied in the field of agriculture

Inactive Publication Date: 2017-09-19
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These reports work on the fermentation formula, fermentation technology and metabolites of Inonotus obliquus, but do not involve the liquid fermentation technology targeting extracellular β-glucan metabolites as indicators, let alone the use of fermentation products as growth regulators. Research Report on Regulating Crop Growth and Improving Economic Value

Method used

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  • Crop growth regulator based on inonotus obliquus, and preparation method and application thereof
  • Crop growth regulator based on inonotus obliquus, and preparation method and application thereof
  • Crop growth regulator based on inonotus obliquus, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of Inonotus obliquus Fruiting Body Extract

[0062] Wild Inonotus obliquus was purchased from the Changbai Mountain area of ​​Jilin Province. The wild Inonotus obliquus was dried at 60°C to constant weight, and then the fungus was crushed and sieved with 120 mesh (sieve hole size: 0.125mm) to obtain the obliquus obliquus. Bacteria powder. Accurately weigh 500 g of Inonotus obliquus powder, and add distilled water according to the ratio of volume to water of 1:40. Boil at 100°C for 2.5 hours, extract twice, combine the extracts, measure the polysaccharide content (the polysaccharide content is determined by the phenol-sulfuric acid method), dilute with water until the polysaccharide content is 10mg / mL, and it is biological bacteria solution No. 1.

Embodiment 2

[0064] Production of Submerged Fermentation Liquid of Inonotus obliquus

[0065]Strain preparation: the Inonotus obliquus bacterial strain (the present invention can also use other Inonotus obliquus bacterial strains) with the preservation number of CGMCC No.10297 is transferred twice with cPDA medium (comprehensive potato dextrose agar medium), each time Cultivate at 25°C for 10 days to obtain mother seed blocks. cPDA medium ( / L): potato 200.0g (leach juice), glucose 20.0g, peptone 0.5g, KH 2 PO 4 3.0g, MgSO 4 .7H 2 O 1.5g, VB 1 10mg, 14g agar, natural pH. Activated first-class medium: 87.5% fine sawdust (passed through a 20-mesh sieve, sieve size: 0.850mm), 20% wheat bran, 1% lime, 0.5% gypsum, 1% sugar, and 60% water content. Put the prepared activated primary seed medium into a 500mL triangular flask, each bottle contains 450g of wet material, seal it with a high-pressure filter film, sterilize at 121°C for 1.5h, cool down, insert 2 pieces of 1cm×1cm mother seed bl...

Embodiment 3

[0069] Biological liquid test work

[0070] 3.1 Purpose of the test

[0071] The purpose of this example is to clarify the safe concentration of biological bacteria liquid sprayed on rice, and the application effect of spraying and flushing.

[0072] 3.2 Test material

[0073] The test materials of this embodiment are biological bacteria liquid No. 1 and biological bacteria liquid No. 2.

[0074] 3.3 Experimental design

[0075] Using the test method of large-scale comparison, take 0-20cm soil layer before site preparation to measure the background value of soil nutrients; dynamically measure the growth characteristics of rice; at the maturity stage, each treatment randomly selects a place with uniform growth, and harvests 1m 2 Conduct test production.

[0076] 3.3.1 Experimental design of spraying:

[0077] Large area comparative test, each treatment 100m 2 , without repetition, spraying at tillering stage and booting stage respectively. Three application gradients of ...

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Abstract

The invention provides a plant (such as a crop) growth regulator. The plant growth regulator contains a polysaccharide-containing active ingredient which can effectively regulate plant growth, and the polysaccharide-containing active ingredient contains the polysaccharide-containing active ingredient sourced from Inonotus obliquus (Fr.)Pilat. The polysaccharide content of the active ingredient in the plant growth regulator is 0.1-100mg/mL. The polysaccharide active ingredient sourced from the inonotus obliquus contains an inonotus obliquus extracting solution or fermentation liquor. The plant growth regulator also can contain active ingredients of additional edible and pharmaceutical fungi, and the edible and pharmaceutical fungi include one or more selected from ganoderma lucidum, mushroom, hericium erinaceus, cordyceps sinensis, poria cocos, polyporus umbellatus, phellinus igniarius, agaric and coriolus versicolor. The invention also provides a method for preparing the plant growth regulator and application of the plant growth regulator.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to a crop growth regulator, in particular to a crop growth regulator based on Inonotus obliquus, a preparation method of the crop growth regulator and an application thereof. Background technique [0002] Inonotus obliguus (Fr.) Pilat is a rare medicinal fungus. Inonotus obliquus, also known as chaga and birch tears, is mainly distributed in Finland, Poland, Russia, Japan and my country. In my country, it is mainly distributed in Heilongjiang and Jilin. Inonotus obliquus rarely forms fruiting bodies in the wild environment, and the fruiting bodies grow slowly. It has medicinal value only when it grows on living birch trees for 10 to 15 years, and only one tree grows on every 20,000 birch trees. Inonotus obliquus. Therefore, wild resources of Inonotus obliquus are extremely rare. With the development of medicinal uses in recent years, the wild resources of Inonotus obliquus have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/16A01P21/00
CPCA01N43/16A01N63/30Y02A40/10
Inventor 韩增华高娃张丕奇戴肖东马庆芳孔祥辉姜宏宇张介驰于德水王晗丛燕
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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