Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for hydrolyzing intestinal mucosa through protease

A technology of protease hydrolysis and intestinal mucosa, applied in the field of protease hydrolysis of intestinal mucosa, can solve problems such as high cost, no mention of removal method, complicated production process, etc., and achieve the effect of improving recovery rate

Inactive Publication Date: 2017-09-19
南通游龙肠衣食品有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned method is a typical industrial process of preparing and purifying heparin sodium, the production process is complicated, and the cost is high, and there is no mention of the removal method of the main impurity, the structural analog chondroitin polysulfate of heparin sodium

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Dissolve 50g of crude heparin sodium in 100mL of 1.2% NaCl solution by mass percentage, stir mechanically until the heparin sodium is completely dissolved, add 1m01 / L NaOH to the solution until precipitation occurs, let it stand for 1 hour, and filter to remove the insoluble matter to obtain Filter lm01 / L NaOH to adjust the pH to 9.5, add 3mLlm01 / L H2O2, react at 10°C for 2.5 hours. The temperature of the reaction solution was raised to 30°C, and then 3 mLlm01 / LH202 was added every 3.5 hours to maintain the pH at 6.8, and the reaction was carried out for 21 hours after feeding. Add 5g of activated carbon to the reaction solution, stir it magnetically, and filter out the insolubles to obtain the filtrate; balance the Sephadex G— 25 column with a mass percent concentration of 4% NaCl solution, the sample layer is 15% of the column bed volume, and the column diameter / height 1:5, pass the filtrate through the column, elute with 4% NaCl in mass percent concentration, the flow...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for preparing and purifying biopharmaceutical raw materials, which belongs to the field of bioengineering, in particular to a protease hydrolysis process for intestinal mucosa. The purpose of the present invention is to provide a process for protease hydrolysis of intestinal mucosa in order to improve the deficiencies of the prior art; the invention provides a simple low-molecular-weight heparin sodium preparation and purification technology. About 5KDa, the potency is not less than 180U / mg, which is higher than the 170U / mg required by the Chinese Pharmacopoeia, and it is qualified according to the Chinese Pharmacopoeia's heparin sodium testing standard.

Description

technical field [0001] The invention relates to a process for hydrolyzing intestinal mucosa with protease in the field of bioengineering. Background technique [0002] Heparin sodium is an anion-rich mucopolysaccharide sulfate, which is produced by mast cells and basophils in animals. It is originally extracted from the liver, so it is named heparin. Clinically used heparin is a sodium (lithium) salt extracted from animal intestines, lung mucosa, and blood vessel walls. Heparin sodium (lithium) has various biological functions such as anticoagulation, anti-inflammation, and anti-cancer, but long-term use There will be side effects such as bleeding and fractures. In the early 1980s, low molecular weight heparin sodium (LMWH, (8000Da) was found to have stronger activity and less side effects, and became the main clinical anticoagulant drug. [0003] At present, more than 10 kinds of LMWH have come out one after another abroad, and the world's four major pharmaceutical comp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0069
Inventor 陈文龙张宏宋磊
Owner 南通游龙肠衣食品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com