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Low-cost production method for dermatan sulfate

A low-cost technology of dermatan sulfate, applied in the field of biochemistry, can solve the problems of long production cycle, high cost of raw materials, and high production process costs, and achieves the effects of improving yield and controlling oxidative inactivation

Inactive Publication Date: 2019-06-18
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the raw material that these methods adopt is soft-shelled turtle cartilage or bovine nasal cartilage mostly, and raw material cost is higher, through complicated procedure, production cycle is long, and the single product dermatan sulfate that obtains, its purity can reach 95.2%, and the whole production process is old higher cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dissolve 50g of crude heparin sodium into drinking water, stir for 5 hours, and dissolve it completely; adjust the pH of the solution to 7.0 with hydrochloric acid solution, then add 5g / 100 million units of pepsin when the temperature rises to 50°C, and then add 5g Trypsin per 100 million units, keep warm for 2 hours; heat the enzymolysis solution to 85°C within 30 minutes, stand still for 10 minutes, stir, and then wait until the temperature drops to 50°C, adjust the pH to 10.0 with 1mol / L sodium hydroxide solution, Stand still for 20 hours, separate layers, filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugate after centrifugation; the supernatant and centrifugate are adsorbed with weakly basic ion exchange resin, and 0.3g / L of sodium chloride was eluted and eluted twice in succession. Take the eluate and adjust the pH to 10.0 with 2mol / L sodium hydroxide solutio...

Embodiment 2

[0018] Dissolve 50g of crude heparin sodium into drinking water, stir for 7 hours, and dissolve it completely; adjust the pH of the solution to 6.0 with hydrochloric acid solution, then add 13g / 100 million units of pepsin when the temperature rises to 53°C, and then add 16g Trypsin per 100 million units, keep warm for 3 hours; heat the enzymolysis solution to 87°C within 36 minutes, stand still for 20 minutes, stir, and then wait until the temperature drops to 53°C, adjust the pH to 11.0 with 2mol / L sodium hydroxide solution, Stand still for 26 hours, separate layers, filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugate after centrifugation; the supernatant and centrifugate are adsorbed with weakly basic ion exchange resin, and 0.36g / L of sodium chloride was eluted and eluted twice in succession. Take the eluate and adjust the pH to 11.0 with 4mol / L sodium hydroxide solu...

Embodiment 3

[0020] Dissolve 50g of crude heparin sodium into drinking water, stir for 10 hours, and dissolve it completely; adjust the pH of the solution to 8.0 with hydrochloric acid solution, then add 15g / 100 million units of pepsin when the temperature rises to 55°C, and then add 25g Trypsin per 100 million units, keep warm for 4 hours; heat the enzymolysis solution to 85-90°C within 40 minutes, stand still for 30 minutes, stir, and then adjust it with 1-3mol / L sodium hydroxide solution when the temperature drops to 55°C PH to 12.0, stand still for 30 hours, separate layers, filter, leave the supernatant for precipitation, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugate after centrifugation; the supernatant and centrifugation are adsorbed with weakly basic ion exchange resin, With 0.4g / L sodium chloride elution, continuous elution twice. Take the eluent and adjust the pH to 12.0 with 2-6mol / L sodium hydroxide solution, then ...

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Abstract

Belonging to the field of biochemistry, the invention discloses a low-cost production method for dermatan sulfate. The method mainly includes: enzymolysis, ion exchange resin exchange, ethanol purification and the like. Heparin sodium is a by-product of dermatin sulfate production, and the two products can be produced simultaneously, thus solving the high cost problem in the dermatin sulfate production process at present. The method has the advantages of simple process, easy operation and cost saving, and is suitable for industrial production.

Description

technical field [0001] The method description relates to a low-cost method for producing raw materials, which belongs to the field of biochemistry, specifically a method for simultaneously producing heparin sodium and simultaneously producing dermatan sulfate. Background technique [0002] Dermatan sulfate (DS) is a disaccharide polymer, which is the most widely distributed extracellular matrix (extra cell ularmatrix, ecm) glycosaminoglycan in animals, and is the main component of proteoglycans in blood vessel walls. [0003] DS is a kind of glycosaminoglycan, a polysaccharide composed of repeating disaccharide units connected by D-glucuronic acid and N-acetylgalactosamine with β-1,4-glycosidic bonds, and in N-acetyl Sulfation occurs on the C-4 or C-6 hydroxyl of galactosamine. Due to the sulfuric acid group, the entire cs molecule presents a strong negative charge, and it is easy to covalently combine with proteins to form proteoglycans, which is also an important basis fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C08B37/10
Inventor 刘乃山迟培升周晓娜
Owner QINGDAO JIULONG BIO PHARMA
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