Method for improving yield of heparin sodium

A technology of heparin sodium and crude heparin, which is applied in the field of preparation of high-purity and high-yield high-quality heparin sodium, can solve the problems of affecting the activity of heparin sodium, not suitable for medicinal use, and not suitable for batch production, and achieve the effect of controlling oxidative inactivation

Inactive Publication Date: 2014-05-21
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological enzymatic method mainly produces heparin sodium by enzymatic degradation. The method is mild, but the cost is high, and it is not suitable for batch production. Another chemical cracking method has a large yield, but the activity of heparin sodium will be affected during the process, and it is not suitable for medicinal use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Dissolve 50 g of crude product heparin sodium into drinking water, stir for 5 hours, and dissolve it completely;

[0024] (2) Use hydrochloric acid solution to adjust the pH to 7.0, then add 5 g / 100 million units of pepsin when the temperature is raised to 50 ° C, then add 10 g / 100 million units of trypsin, and keep warm for 2 hours;

[0025] (3) Heat the enzymolysis solution to 85°C within 30 minutes, stand still for 10 minutes, stir, and then wait for the temperature to drop to 50°C, adjust the pH to 10.0 with 2mol sodium hydroxide solution, stand still for 20 hours, separate layers, and siphon the supernatant , filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifuge after centrifugation;

[0026] (4) When the temperature of the supernatant and the centrifugate drops to 20°C, add 25g / L sodium chloride, stir to dissolve, adjust the pH to 10.0 with hydrochloric acid...

Embodiment 2

[0036] (1) Dissolve crude heparin sodium into drinking water, stir for 5-10 hours, and dissolve it completely;

[0037] (2) adjust the pH of the dissolving solution to 6.5 with hydrochloric acid solution, then add 7 g / 100 million units of pepsin when the temperature is raised to 52, then add 15 g / 100 million units of pancreatin, and keep warm for 3 hours;

[0038] (3) Heat the enzymolysis solution to 87°C within 35 minutes, stand still for 20 minutes, stir, and then wait until the temperature drops to 52°C, adjust the pH to 11.0 with 4mol sodium hydroxide solution, stand still for 25 hours, separate layers, and siphon up Clear, filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugal liquid after centrifugation;

[0039] (4) When the temperature of the supernatant and the centrifugate drops to 25°C, add 25g / L25g / L sodium chloride, stir to dissolve, adjust the pH to 11.0 with h...

Embodiment 3

[0049] (1) Dissolve crude heparin sodium into drinking water, stir for 10 hours, and dissolve it completely;

[0050] (2) Use hydrochloric acid solution to adjust the pH to 8.0, then add 10 g / 100 million units of pepsin when the temperature is raised to 50-55 ° C, then add 20 g / 100 million units of pancreatin, and keep warm for 4 hours;

[0051] (3) Heat the enzymolysis solution to 90°C within 40 minutes, stand still for 30 minutes, stir, and then wait until the temperature drops to 55°C, adjust the pH to 12.0 with 6mol sodium hydroxide solution, stand still for 30 hours, separate layers, and siphon up Clear, filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugal liquid after centrifugation;

[0052] (4) When the temperature of the supernatant and the centrifugate drops to 30° C., add 30 g / L sodium chloride, stir to dissolve, adjust the pH to 12.0 with hydrochloric acid, add...

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Abstract

The invention relates to a method for improving yield of heparin sodium, and discloses an extraction technology for preparing high purity and high yield heparin sodium, belonging to chemical field. The method mainly comprises steps of extracting by acetone and purifying by ethyl alcohol. By adopting the method, the problem that oversulfated chondroitin sulfate is mixed in the existing production process of heparin sodium is solved, the cost is saved, the purity of heparin sodium is improved, and the method is simple, is easy to operate, and is applicable to industrial production.

Description

technical field [0001] The description of this method relates to a method for extracting and purifying raw materials, which belongs to the chemical field, and is specifically a method for preparing high-purity and high-yield high-quality heparin sodium. Background technique [0002] Heparin sodium is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle. It is a mucopolysaccharide substance and exists in the form of heparin sodium-protein complexes in animals. In medicine, heparin sodium has anticoagulant effect both in vivo and in vitro, and can prolong coagulation time, prothrombin time and thrombin time. Clinically, it has the functions of anticoagulation, lowering blood lipid, protecting endothelial cells, anti-platelet accumulation and release, promoting fibrinolysis, inhibiting the proliferation of arterial smooth muscle cells, reducing blood viscosity and anti-inflammation. [0003] my country is the largest producer of hepari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 刘乃山周晓娜迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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