Method for improving yield of heparin sodium
A technology of heparin sodium and crude heparin, which is applied in the field of preparation of high-purity and high-yield high-quality heparin sodium, can solve the problems of affecting the activity of heparin sodium, not suitable for medicinal use, and not suitable for batch production, and achieve the effect of controlling oxidative inactivation
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Embodiment 1
[0023] (1) Dissolve 50 g of crude product heparin sodium into drinking water, stir for 5 hours, and dissolve it completely;
[0024] (2) Use hydrochloric acid solution to adjust the pH to 7.0, then add 5 g / 100 million units of pepsin when the temperature is raised to 50 ° C, then add 10 g / 100 million units of trypsin, and keep warm for 2 hours;
[0025] (3) Heat the enzymolysis solution to 85°C within 30 minutes, stand still for 10 minutes, stir, and then wait for the temperature to drop to 50°C, adjust the pH to 10.0 with 2mol sodium hydroxide solution, stand still for 20 hours, separate layers, and siphon the supernatant , filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifuge after centrifugation;
[0026] (4) When the temperature of the supernatant and the centrifugate drops to 20°C, add 25g / L sodium chloride, stir to dissolve, adjust the pH to 10.0 with hydrochloric acid...
Embodiment 2
[0036] (1) Dissolve crude heparin sodium into drinking water, stir for 5-10 hours, and dissolve it completely;
[0037] (2) adjust the pH of the dissolving solution to 6.5 with hydrochloric acid solution, then add 7 g / 100 million units of pepsin when the temperature is raised to 52, then add 15 g / 100 million units of pancreatin, and keep warm for 3 hours;
[0038] (3) Heat the enzymolysis solution to 87°C within 35 minutes, stand still for 20 minutes, stir, and then wait until the temperature drops to 52°C, adjust the pH to 11.0 with 4mol sodium hydroxide solution, stand still for 25 hours, separate layers, and siphon up Clear, filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugal liquid after centrifugation;
[0039] (4) When the temperature of the supernatant and the centrifugate drops to 25°C, add 25g / L25g / L sodium chloride, stir to dissolve, adjust the pH to 11.0 with h...
Embodiment 3
[0049] (1) Dissolve crude heparin sodium into drinking water, stir for 10 hours, and dissolve it completely;
[0050] (2) Use hydrochloric acid solution to adjust the pH to 8.0, then add 10 g / 100 million units of pepsin when the temperature is raised to 50-55 ° C, then add 20 g / 100 million units of pancreatin, and keep warm for 4 hours;
[0051] (3) Heat the enzymolysis solution to 90°C within 40 minutes, stand still for 30 minutes, stir, and then wait until the temperature drops to 55°C, adjust the pH to 12.0 with 6mol sodium hydroxide solution, stand still for 30 hours, separate layers, and siphon up Clear, filter, leave the supernatant to be precipitated, put the lower layer of sediment in a centrifuge for centrifugation, and leave the upper layer of centrifugal liquid after centrifugation;
[0052] (4) When the temperature of the supernatant and the centrifugate drops to 30° C., add 30 g / L sodium chloride, stir to dissolve, adjust the pH to 12.0 with hydrochloric acid, add...
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