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Technology for preparing high purity low molecular weight heparin sodium

A low-molecular-weight heparin sodium and high-purity technology is applied in the preparation and purification of high-purity low-molecular-weight heparin sodium and biopharmaceutical raw materials. The effect of recovery

Inactive Publication Date: 2015-04-01
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method is a typical industrial process for preparing and purifying heparin sodium, the production process is complex and the cost is high, and there is no mention of the removal method of the main impurity, the structural analogue of heparin sodium chondroitin polysulfate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention is further explained below in conjunction with examples, but the implementation cases do not limit the present invention in any form.

[0021] Dissolve 50 grams of crude heparin sodium in 150 mL of a 1.6% NaCl solution by mass percentage, mechanically stir until the heparin sodium is completely dissolved, add 1.2 mol / L KOH to the solution until precipitation occurs, let it stand for 1.5 hours, and filter to remove incompatible substances to obtain Filtrate; adjust pH to 10.5 with 1.2mol / L KOH, add 7.5mL 5moi / L of H 2 o 2 , 30°C, reacted for 3.5 hours. The temperature of the reaction solution was raised to 50 °C, and 7.5 mL of 3 mol / L H was added every 3 hours thereafter. 2 o 2 , maintain pH 7.5, and react for 15 hours after feeding. Add 4.5g of bentonite to the reaction solution, stir magnetically, filter to remove insoluble matter, and obtain the filtrate; equilibrate the Sephadex G-25 column with 6% NaCL solution by mass percentage, and the sa...

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Abstract

The invention discloses a technology for preparing high purity low molecular weight heparin sodium and belongs to the field of biological engineering. The technology mainly comprises the steps of salting out for purification, material supplement, heating-up, oxidation, gel elution, ion exchange, ethanol precipitation, vacuum drying and the like. The technology solves the problems that heparin sodium analogues are difficult to remove, the purity cannot meet medicinal requirements, and the preparation technology is complicated and the like at present. The technology realizes preparation and decoloration of low molecular weight heparin sodium by a material supplement, heating-up and oxidation method, weakly-basic ion exchange resin is used for effectively removing the heparin sodium analogues, a production cycle is shortened, the cost is saved, and the technology is simple easy to implement and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing and purifying biomedicine raw materials, which belongs to the field of bioengineering, in particular to a process for preparing high-purity low-molecular-weight heparin sodium. Background technique [0002] Heparin sodium is an anion-rich mucopolysaccharide sulfate, which is produced by mast cells and basic granulocytes in animals. It is originally extracted from the liver, so it is named heparin. Clinically used heparin is a sodium salt extracted from animal intestines, lung mucosa, and blood vessel walls. Heparin sodium has various biological functions such as anticoagulation, anti-inflammation, and anti-cancer, but long-term use will cause bleeding, fractures, etc. Wait for negative effects. In the early 1980s, low-molecular-weight heparin sodium was found to have stronger activity and less side effects, and became the main clinical anticoagulant drug. [0003] At present, more than 10 kinds of LMWH ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 刘冠男尹倩夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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