Proteinase K freeze drying technology

Abstract

The invention discloses a proteinase K freeze drying technology which comprises the following steps: pretreating proteinase K to be freeze dried, performing pre-freezing treatment on the proteinase K, then heating to a second temperature to quickly drying for the first time and then heating temperature to a third temperature to desorption drying; then reducing pressure, cooling to the second temperature, then haeting to the third temperature and performing special technologies of secondary drying and desorption drying. Compared with the prior art, freeze drying time of the proteinase K freeze drying technology is shortened to 20 to 26 hours; furthermore, a freeze dried product prepared in the proteinase K freeze drying technology has appearance shape meeting a standard, can redissolve quickly and has good stability; proteinase K activity cannot decrease after the proteinase K is stored in normal temperature for many years.

Description

technical field [0001] The invention relates to the field of freeze-drying, in particular to the freeze-drying process of proteinase K. Background technique [0002] Proteinase K is a highly active protease of the subtilisin class used for the general degradation of proteins in biological samples. Purified from Tritirachium album limber. According to the data, the enzyme has two ca2+ binding sites, which are a certain distance away from the active center of the enzyme, and have no direct relationship with the catalytic mechanism. However, if Ca2+ is removed from the enzyme, about 80% of the catalytic activity is lost due to long-range structural changes, but the remaining activity is usually sufficient to degrade proteins that normally contaminate acid preparations. [0003] As a protease, proteinase K can digest and degrade other proteins, but it is also a protein itself. Like most proteins, its activity and stability are also affected by factors such as temperature, pH ...

AI Technical Summary

Problems solved by technology

However, the existing proteinase K freeze-drying process is still insufficient, and it takes too long to affect the production of proteinase K freeze-dried products.