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Multi-labeling immunohistochemical staining kit for tumor emboli

A technology of immunohistochemistry and staining reagents, which is applied in the direction of material analysis by observing the influence of chemical indicators, preparation of test samples, analysis by making materials undergo chemical reactions, etc., which can solve the early judgment of capillary cancer embolism Difficult, difficult to find and other problems, to achieve the effect of less dyeing steps, short experiment time and high stability

Inactive Publication Date: 2017-09-19
FUZHOU MAIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to judge early tumor thrombi in capillaries, venules, and lymphatic vessels in HE-stained sections.
One is that the early tumor thrombus is small and difficult to be found; the other is that the infiltrating cancer nest shrinks and forms a gap with the surrounding stroma, which is difficult to distinguish from the intravascular tumor thrombus

Method used

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  • Multi-labeling immunohistochemical staining kit for tumor emboli

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Embodiment 1

[0029] The research object was formaldehyde-fixed-paraffin-embedded early invasive urothelial carcinoma, and the multi-label immunohistochemical staining steps for tumor thrombi were as follows:

[0030] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0031] (2) Conventional xylene dewaxing for 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0032] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0033] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0034] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD34, D2-40 and Cytokeratin (Pan) mixed primary antibody dropwise, incubate at room t...

Embodiment 2

[0040] The research object is formaldehyde-fixed-paraffin-embedded invasive breast cancer tissue, and the steps of multi-label immunohistochemical staining of tumor thrombi are as follows:

[0041] (1) The tissue slices were dry-baked in a 67°C incubator for 2 hours.

[0042] (2) Conventional xylene dewaxing for 3 times, 6 minutes each time, hydration in 100%, 100%, 95%, 85% gradient ethanol, 3 minutes each time, and finally rinsed with tap water.

[0043] (3) High-pressure repair in citric acid antigen retrieval solution of pH 6.0 for 1.5-2 minutes, naturally cool to room temperature, rinse with tap water, and rinse with PBS for 3×3 minutes.

[0044] (4) Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0045] (5) Block with normal animal serum for 10 minutes, shake off excess serum without washing, add CD31, D2-40 and Cytokeratin (Pan) mixed primary antibody dropwise, incubate at room tempera...

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Abstract

The invention belongs to the technical field of immunohistochemistry, relates to a multi-labeling immunohistochemical staining kit for tumor emboli to display tumor emboli in blood vessels and lymph vessels and is intended to solve the problem that it is difficult to clearly distinguish intravascular tumor emboli and mesenchymal invasive carcinoma nest in HE (hematoxylin and eosin) staining piece. The multi-labeling immunohistochemical staining kit for tumor emboli comprises a mixed liquid of antibodies A, B and C; the antibody A is CD34 or CD31, the antibody B is Podoplanin Protein or D2-40, and the antibody is Cytokeratin (Pan); the multi-labeling immunohistochemical staining kit for tumor emboli is used for, on a same section, displaying vascular endothelial cells, lymphatic endothelial cells and cancer cells and is capable of clearly displaying cancer cells in vessels, particularly minor tumor emboli, so that over-diagnosis or insufficient diagnosis of intravascular tumor emboli due to artificial judgment errors can be avoided.

Description

technical field [0001] The invention belongs to the technical field of immunohistochemistry, in particular, the invention relates to a tumor embolus multilabel immunohistochemical staining kit for displaying tumor thrombi in blood vessels and lymphatic vessels. technical background [0002] Different stages of cancer have different treatment strategies, so its pathological staging is of great significance for clinical treatment and prognosis. The main method of pathological staging of cancer is to observe the infiltration range of cancer cells in tissues and organs under a microscope, whether there is tumor thrombus in the blood vessel, whether there is lymph node metastasis, and combine with clinical imaging to judge whether there is distant organ metastasis, and finally determine the cancer cell. Therefore, intravascular tumor thrombus is an important indicator of pathological observation. However, it is difficult to judge early tumor thrombi in capillaries, venules, and ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/535G01N21/78G01N1/30G01N1/36
CPCG01N33/574G01N1/30G01N1/36G01N21/78G01N33/535G01N33/57407G01N33/57415G01N2800/34G01N2800/365G01N2800/56
Inventor 郑智勇杨清海
Owner FUZHOU MAIXIN BIOTECH CO LTD
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