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Culture medium for promoting rapid growth of oyster mushroom liquid mycelia and culture method

A technology of liquid culture and culture method, which is applied in the field of culture medium and culture to promote the rapid growth of Pleurotus ostreatus liquid mycelia, can solve the problems of no application, complicated medium formula, and inability to effectively reduce factory operation costs, so as to promote rapid growth of Pleurotus ostreatus The effect of growth

Pending Publication Date: 2017-09-22
INST OF PLANT NUTITUION & RESOURCE ENVIRONMENT HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, the existing problems in the prior art are: in production, the application of liquid strains of Pleurotus ostreatus is seldom used, especially in the factory production mode.
At the same time, the formula of the existing culture medium is relatively complicated, and there are many types of substances contained in the formula, and the production process is also relatively complicated.
The existing technology cannot make the liquid strain of Pleurotus ostreatus grow rapidly in a relatively short period of time, and cannot effectively reduce the reduction of factory operation costs

Method used

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  • Culture medium for promoting rapid growth of oyster mushroom liquid mycelia and culture method

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Embodiment 1

[0033] The cultivation method that the embodiment of the present invention provides to promote the rapid growth of Pleurotus ostreatus liquid culture hyphae comprises the following steps:

[0034] Strain activation culture: take out the oyster mushroom strains preserved at low temperature (4°C) or liquid nitrogen, inoculate them on the potato-dextrose-agar (PDA) plate medium, carry out 25°C activation culture for 8d to 10d, and repeat the activation twice; Then use a puncher (diameter 5mm) to get the bacterial block on the plate and inoculate the bacterial block in the center of the plate containing the PDA medium, and cultivate it at 25°C for 10 days; then use the same puncher to get the bacterial block;

[0035] Preparation of culture medium: 200g of peeled potatoes, cut into small pieces of about 0.75cm, put into a pot filled with 800mL of boiled deionized water, boil for 10min-15min; then filter with 4 layers of gauze, add 20g of Deionized water was used to make it complet...

Embodiment 2

[0038] The cultivation method that the embodiment of the present invention provides to promote the rapid growth of Pleurotus ostreatus liquid culture hyphae comprises the following steps:

[0039] Strain activation culture: take out the oyster mushroom strains stored at low temperature (4°C) or liquid nitrogen, inoculate them on the potato-glucose-agar PDA plate medium, carry out 25°C activation culture for 8d-10d, repeat the activation twice; then use Use a hole punch (5 mm in diameter) to pick out the bacterial block on the plate and inoculate the bacterial block in the center of the plate containing the PDA medium, and culture it at 25°C for 10 days; then use the same punch to get the bacterial block;

[0040]Preparation of culture medium: 200g of peeled potatoes, cut into small pieces of about 0.5cm, put into a pot filled with 800ml of boiled deionized water, boil for 10min-15min; then filter with 4 layers of gauze, add 20g of Deionized water was used to make it completely...

Embodiment 3

[0043] The cultivation method that the embodiment of the present invention provides to promote the rapid growth of Pleurotus ostreatus liquid culture hyphae comprises the following steps:

[0044] Strain activation culture: take out the oyster mushroom strains stored at low temperature (4°C) or liquid nitrogen, inoculate them on the potato-glucose-agar PDA plate medium, carry out 25°C activation culture for 8d-10d, repeat the activation twice; then use Use a hole punch (5 mm in diameter) to pick out the bacterial block on the plate and inoculate the bacterial block in the center of the plate containing the PDA medium, and culture it at 25°C for 10 days; then use the same punch to get the bacterial block;

[0045] Preparation of culture medium: 200g of peeled potatoes, cut into small pieces of about 1cm, put into a pot filled with 800mL of boiled deionized water, boil for 10min-15min; then filter with 4 layers of gauze, add 20g of glucose to the filtrate , make it dissolve comp...

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Abstract

The invention belongs to the technical field of edible mushroom cultivation and discloses a culture medium for promoting the rapid growth of oyster mushroom liquid mycelia and a culture method. The culture method comprises the following steps: carrying out activating culture on strains, producing the culture medium, culturing the mycelia and determining mycelium biomass. The step of culturing the mycelia comprises following steps of carrying out punching oyster mushroom mycelia growing on a flat plate by a punching device, then inoculating a potato-dextrose-agar (PDA) liquid culture medium which contains 0.1 percent to 1.0 percent of peanut oil and has no agar and carrying out shake-flask culture. After 0.1 percent to 1.0 percent of the peanut oil is added, the color of the mycelia becomes strong white, branches of the mycelia are increased and fine and small mycelium pellets are increased; the dry weight of the mycelia is 109.02 percent to 331.96 percent higher than that of the mycelia which are treated without the peanut oil.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a culture medium and a cultivation method for promoting the rapid growth of liquid hyphae of oyster mushrooms. Background technique [0002] Liquid spawn technology is a commonly used technology in the production of edible fungi, especially in the industrial production of edible fungi. It has many advantages: first, the production process is simplified, there is no distinction between mother species, original species and cultivated species, the process is simple, and time and cost are saved ; Second, the strains are more pure, less polluted by miscellaneous bacteria, the germination points are scattered, and the germination is fast; third, the inoculation speed is fast and the cost is low; fourth, the production cycle is short, and the mushrooms are neat, suitable for large-scale production, no season Fifth, it is convenient to replace the bacteria, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 孔维威康源春袁瑞奇孔维丽张玉亭韩玉娥刘芹胡素娟宋志波段亚魁徐柯崔筱
Owner INST OF PLANT NUTITUION & RESOURCE ENVIRONMENT HENAN ACADEMY OF AGRI SCI
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