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Application of Pillar[5]arene as Molecular Sensor for Colorimetric-Fluorescent Recognition of Methionine in Pure Aqueous Phase

A technology of molecular sensor and fluorescence recognition, applied in fluorescence/phosphorescence, instruments, analysis materials, etc., can solve problems such as insolubility

Inactive Publication Date: 2020-06-12
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the pillar [5] aromatics only have two symmetrical long alkyl chains, they cannot be dissolved in water, and can only be identified after being dissolved in an organic solvent.

Method used

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  • Application of Pillar[5]arene as Molecular Sensor for Colorimetric-Fluorescent Recognition of Methionine in Pure Aqueous Phase
  • Application of Pillar[5]arene as Molecular Sensor for Colorimetric-Fluorescent Recognition of Methionine in Pure Aqueous Phase
  • Application of Pillar[5]arene as Molecular Sensor for Colorimetric-Fluorescent Recognition of Methionine in Pure Aqueous Phase

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Embodiment Construction

[0028] The method for colorimetric-fluorescent detection of methionine by the sensor molecule P5 of the present invention will be further described through specific examples below.

[0029] Synthesis of Pillar[5]arene P5: According to literature: Y.Ma,X. Ji,F. Xiang, X. Chi, C. Han, J. He,Z. Abliz, W. Chen, F. Huang, Chem. Commun. 47 (2011) 12340 Synthesis.

[0030] Column [5] Arene P5 fluorescence detection Met: In the aqueous solution of the sensor molecule P5, add Ala, Arg, Asp, Cys, Gln, Glu, His, Ile, Gly, Asn, Leu, Lys, Met, Phe, Pro, Ser , Thr, Trp, Tyr and Val in aqueous solution (4 × 10 -3 mol L -1 ), if the P5 solution produces light yellow-green fluorescence, it means that Met was added; if the fluorescence of the P5 solution does not change significantly, it means that other amino acids were added.

[0031] Pipette column [5] aromatics sensor supramolecular aqueous solution (2 × 10 -3 mol / L) in a series of drip plates, add Ala, Arg, Asp, Cys, Gln, Glu, His, Il...

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Abstract

The invention provides an application method of column [5] arene as a molecular sensor for high-selectivity and high-sensitivity colorimetric-fluorescent identification of methionine in a pure water phase, belonging to the field of molecular detection. The experiments show that in column [5] arene water solution, only the added methionine can generate subjective and objective body complexation with a P5 subject to cause a phenomenon of changing the achromatic color of solution fluorescence into pistac, so that the colorimetric-fluorescent specificity identification of the methionine is realized, whereas other amino acid cannot cause interference to P5 specificity identification. In addition, P5 has high sensitivity to methionine detection, and the lowest detection limit is that LOD is equal to 6.328 multiplied by 10<7>M. Therefore, the molecular sensor is a super molecular sensor for high-selectivity and high-sensitivity detection of the methionine in water.

Description

technical field [0001] The invention relates to the application of pillar[5]arene in the field of molecular detection, in particular to the application of pillar[5]arene as a molecular sensor for colorimetric-fluorescence specific recognition of methionine in pure water phase, and belongs to the field of molecular detection. Background technique [0002] Methionine is one of the essential amino acids that constitute the human body. It participates in protein synthesis and is a sulfur-containing essential amino acid. The organism must convert the D-type into the L-type in the body before it can be used by the body. It is closely related to the metabolism of various sulfur-containing compounds in organisms. In the living body, it first accepts adenosyl group from ATP to become S-adenosylmethionine (active methionine) and then undergoes methyl transfer. Homocysteine ​​that loses its methyl group is converted to cysteine ​​by cystathionine. When methionine is lacking, it will ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/78G01N21/79
CPCG01N21/64G01N21/6428G01N21/643G01N21/78G01N21/79G01N2021/6417G01N2021/6443G01N2021/6495
Inventor 林奇关晓文郑峰刘露毛鹏鹏樊彦青魏太保张有明姚虹
Owner NORTHWEST NORMAL UNIVERSITY