Quick analysis method of content of theanine in fresh tea leaf sample

A rapid analysis, theanine technology, applied in the field of analytical chemistry, can solve the problems of high cost, slow speed, low efficiency, etc., and achieve the effect of high cost and fast analysis speed

Active Publication Date: 2017-09-26
SUZHOU VOCATIONAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a rapid analysis method for theanine content in fresh tea samples, and its purpose is to solve the problems of slow speed, low efficiency, high cost, and difficulty in basic application and popularization existing in the existing tea theanine analysis method

Method used

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  • Quick analysis method of content of theanine in fresh tea leaf sample
  • Quick analysis method of content of theanine in fresh tea leaf sample
  • Quick analysis method of content of theanine in fresh tea leaf sample

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Embodiment

[0034] Example: A rapid analysis method for theanine content in fresh tea samples

[0035] Described rapid analysis method comprises following two parts:

[0036] The first part is to prepare the solution, and then use high performance liquid chromatography-fluorescence detection method to establish a standard curve of the tested theanine with known gradient concentration; the establishment of the standard curve consists of the following steps:

[0037] Step (1), prepare theanine standard, and then prepare 0.14 mol / L phosphate buffer, 0.4 mol / L borate buffer, 1mg / mL AQC derivative solution, and 6 concentrations of theanine Acid standard working solution, the concentrations of theanine standard working solution are 1μmol / L, 5μmol / L, 50μmol / L, 500μmol / L, 1000μmol / L, 1250μmol / L;

[0038] The preparation method of 0.14 mol / L phosphate buffer solution is: weigh 19.0g of sodium acetate trihydrate and 1.72g of triethylamine dissolved in 1000mL of water, adjust the pH to 5.05 with ph...

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Abstract

The invention discloses a quick analysis method of content of a theanine in fresh tea leaf sample. The quick analysis method is characterized by including: smashing and well mixing the fresh tea leaf sample, using ultrapure water as an extraction solvent, extracting at 90 DEG C for 20min, centrifugally filtering, using 6-aminoquinoline-N-hydroxysuccinimido carbamate as a derivating agent for pre-column derivatization, using an XBridge C18 chromatographic column for gradient elution, and using a high performance liquid chromatography-fluorescence detector for separating detection. The method is quick and simple in sample separation, low in cost, good in precision, accuracy, stability and linear relation, quick and sensitive in whole analysis process, high in repeatability and suitable for quick analysis of teanine in the fresh tea leaf sample.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a rapid analysis method for theanine content in fresh tea samples. Background technique [0002] Tea is one of the three major natural beverages in the world. It has a unique aroma, rich nutrition and health benefits, and is deeply loved by consumers. Tea is rich in amino acids, which are important components of the taste of tea and directly affect the quality of tea. Among them, theanine is a unique amino acid in tea, accounting for more than 50% of the total amino acids in tea. Theanine is safe and non-toxic, and has various biological functions such as lowering blood pressure, anti-fatigue, and anti-tumor. With the discovery of theanine's physiological functions and medical value, theanine has been widely used in medical treatment, health care, food, beverages and fine chemicals. and other fields are widely used. The accurate quantitative analysis of theanine is of great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N21/64
CPCG01N21/64G01N30/02G01N2021/6417
Inventor 张丽
Owner SUZHOU VOCATIONAL UNIV
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