PCT (Procalcitonin) fluorescent microsphere immunochromatographic assay reagent card and preparation method thereof

Abstract

The invention discloses a PCT (Procalcitonin) fluorescent microsphere immunochromatographic assay reagent card and a preparation method thereof, aims to provide a PCT fluorescent microsphere immunochromatographic assay reagent card which is convenient in operation, and is capable of detecting low-concentration and high-concentration PCT antigens at the same time and of which the detection result is reliable, and belongs to the technical field of bioassay. The PCT fluorescent microsphere immunochromatographic assay reagent card is characterized is that the PCT fluorescent microsphere immunochromatographic assay reagent card comprises a bottom plate, wherein the bottom plate is sequentially connected with a sample pad, a combination pad, a coating membrane and an absorbing pad; the combination pad is sprayed with two kinds of PCT antibodies labeled by fluorescent microspheres in different particle diameters and fluorescent microsphere-labeled chicken IgY (Immunoglobulin of Yolk); a T-line position of the coating membrane is coated with an anti-PCT antibody, and a C-line position is coated with a goat anti-chicken IgY.

Description

technical field [0001] The invention discloses a detection card, in particular to a PCT fluorescent microsphere immunochromatography detection reagent card, and the invention also relates to a preparation method of the PCT fluorescent microsphere immunochromatography detection reagent card. Background technique [0002] Procalcitonin (PCT) is a small protein with a molecular weight of about 13kDa, which is usually produced by C cells of the thyroid. PCT is now recognized as a major marker of concomitant systemic inflammation and sepsis. [0003] PCT is encoded by the CALC-1 gene and is the precursor of calcitonin. PCT is derived from the precursor of procalcitonin, which consists of 141 amino acids. After removing the signal peptide (1-25 amino acids), PCT containing 116 amino acid residues is obtained. After continuous cleavage, PCT finally forms three molecules , are the N-terminal fragment (N-terminal PCT, 57 amino acid residues), calcitonin (32 amino acid residues) and...

AI Technical Summary

Problems solved by technology

At present, PCT is widely used in hospitals and blood systems as radioimmunoassay (RIA) and enzyme immunoassay (EIA), but there are the following defects in practical application: radionuclide contamination of radioimmunoassay; The activity of the enzyme is easily inactivated, and the macromolecular labeling of the enzyme is easy to affect the spatial structure of the labeled substance

[0008] Chinese patent ZL 201610031801.3 discloses a kit for time-resolved fluorescence quantitative detection of PCT, but the kit has high requirements for the preparation of fluorescent microsphere antibody complexes, low temperature of -80°C and the need for freeze-drying, and the detection sensitivity is low, only 0.1ng / mL, and can only detect serum only, the detection time is long, it takes 20 minutes

Images