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Supercritical carbon dioxide fluid extraction method of astaxanthin from transgenic engineering tomato fruit

A transgenic engineering, carbon dioxide technology, applied in chemical instruments and methods, extraction purification/separation, hydrocarbon purification/separation, etc., to achieve the effect of high extraction rate and safe material use

Active Publication Date: 2019-06-11
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

There is also no report on the supercritical carbon dioxide fluid extraction method of carotenoids in transgenic engineering tomato fruits

Method used

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  • Supercritical carbon dioxide fluid extraction method of astaxanthin from transgenic engineering tomato fruit
  • Supercritical carbon dioxide fluid extraction method of astaxanthin from transgenic engineering tomato fruit
  • Supercritical carbon dioxide fluid extraction method of astaxanthin from transgenic engineering tomato fruit

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Embodiment 1

[0022] Materials and methods

[0023] Materials and equipment:

[0024] Absolute ethanol, 75% ethanol, ethyl acetate, acetone, and n-hexane are domestic analytically pure; isopropanol, acetonitrile, and methanol are German Fisher chromatographically pure; supercritical carbon dioxide fluid extraction device, American Waters (TharSFE); ultra-efficient Liquid chromatograph, Agilent Technologies, USA, 1290Infinity; column, Agilent Technologies, USA, Eclipse plus C18 RRHD 1.8μm; rotary evaporator, Tokyo Rika, EYELE N-1100; analytical balance, Beijing Sartorius Instrument Systems Ltd., TP-213.

[0025] Tomato fruit dry powder: Astaxanthin engineering tomato (Huang Junchao et al. (Huang JC et al., 2013) screened and isolated green algae through genetic engineering methods to obtain a ketogenic enzyme that can catalyze β-carotene and zeaxanthin into astaxanthin in plant cells and hydroxylase genes were transferred into wild-type tomato plants (cv.UC82B), and the obtained transgenic...

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Abstract

Disclosed is a supercritical carbon dioxide fluid extraction method of astaxanthin of genetically-engineered tomato fruit. The supercritical carbon dioxide fluid extraction method is characterized by including: taking dried powder of the engineered tomato fruit of the astaxanthin, adjusting system temperature and pressure to set values and performing 1 hour static extraction after the temperature and pressure of an extraction kettle reach the set values and are stable; turning on a constant-flow pump after completion of static extraction, delivering an entrainer to environment by 1.0g / min, and allowing mixed fluid of the entrainer and carbon dioxide gas to flow into the extraction kettle to start dynamic extraction. The method is safe in material, high in extraction rate and quite suitable for extraction of the astaxanthin of the genetically-engineered tomato fruit. In addition, the genetically-engineered tomato fruit contents lycopene and carotenoid of beta carotene and the like which are high in nutritional value, and the extraction rate of the lycopene is 82% and the extraction rate of the beta carotene is 89% under optimal extraction conditions of the astaxanthin; based technology is laid for genetically-engineered tomato industrialization extraction of the astaxanthin.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a supercritical carbon dioxide fluid extraction method for astaxanthin in genetically engineered tomato fruits, and also provides a supercritical carbon dioxide fluid extraction method for carotenoids in genetically engineered tomato fruits. Background technique: [0002] Astaxanthin is the biologically active substance with the strongest antioxidant activity in nature. With the development of plant genetic engineering technology, the production of astaxanthin with plants, especially economic crops, as bioreactors has become a research hotspot at home and abroad. Astaxanthin It is a ketone carotenoid with a unique molecular structure. It is the biologically active substance with the strongest antioxidant activity in nature. It has the effects of anti-radiation, anti-aging, anti-tumor and prevention of cardiovascular diseases. It has been used in cosmetics, health products...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24C07C7/00C07C7/10C07C11/21
CPCC07C7/00C07C7/10C07C403/24C07C11/21Y02P20/54
Inventor 何明霞黄俊潮
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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