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Bacillus amyloliquefaciens B6 and application thereof

A technology of amylolytic spores and bacilli, which is applied in the field of microorganisms, can solve the problems of pressing sand melons and the difficulty of surviving bio-control bacteria, and achieve the effects of reducing the possibility, good effect, and protecting the roots of watermelons

Active Publication Date: 2017-10-13
BEIFANG UNIV OF NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the soil in Ningxia’s sand-pressed watermelon contains almost no organic matter, and even common soil particles do not exist. The watermelon grows in the sand and gravel artificially moved to the ground, and the bio-control bacteria applied to the ground are difficult to survive. Therefore, The prevention and control of continuous cropping obstacles in Ningxia, Gansu and other places has become a worldwide problem

Method used

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  • Bacillus amyloliquefaciens B6 and application thereof
  • Bacillus amyloliquefaciens B6 and application thereof
  • Bacillus amyloliquefaciens B6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The soil-borne disease biological control matrix of the present embodiment is formed by inoculating the seedling-growing matrix with Bacillus amyloliquefaciens B6, and the effective viable count of the Bacillus amyloliquefaciens B6 of the soil-borne disease biological control matrix is ​​5,000,000 / gram;

[0056] Described seedling-raising matrix comprises the component of following percentage by weight:

[0057] Organic Fertilizer 79.5%

[0058] water 20%

[0059] Binder 0.5%

[0060] Wherein, the binder is hydroxymethyl cellulose.

[0061] The organic fertilizer is composed of peat and fermented animal manure, the weight ratio of the peat to the fermented animal manure is 3:1, the peat is Liaoning peat, and the animal manure is Qinghai plateau sheep manure;

[0062] Described fermentation treatment comprises the steps:

[0063] (1) Water content adjustment: adjust the water content of animal manure to 60wt%;

[0064] (2) Adding an organic decomposing agent: adding...

Embodiment 2

[0073] The difference between this embodiment and embodiment 1 is:

[0074] The effective viable count of Bacillus amyloliquefaciens B6 of the soil-borne disease biological control matrix is ​​6 million / gram

[0075] Described seedling-raising matrix comprises the component of following percentage by weight:

[0076] Organic Fertilizer 69%

[0077] water 30%

[0078] Binder 1%.

[0079] Described fermentation treatment comprises the steps:

[0080] (1) Water content adjustment: adjust the water content of animal manure to 50wt%;

[0081] (2) Adding an organic decomposing agent: adding an organic decomposing agent to the animal manure whose water content has been adjusted and mixing, the amount of the organic decomposing agent added is 0.02wt% of the animal manure whose water content has been adjusted;

[0082] (3) High-temperature sterilization: use the heat of reaction between the organic decomposing agent and animal manure to maintain the temperature of the animal manur...

Embodiment 3

[0085] The difference between this embodiment and embodiment 1 is:

[0086] The effective number of viable bacteria of Bacillus amyloliquefaciens B6 of the soil-borne disease biological control matrix is ​​8 million / gram

[0087] Described seedling-raising matrix comprises the component of following percentage by weight:

[0088] Organic Fertilizer 89.9%

[0089] water 10%

[0090] Binder 0.1%.

[0091] Described fermentation treatment comprises the steps:

[0092] (1) Water content adjustment: adjust the water content of animal manure to 70wt%;

[0093] (2) Adding an organic decomposing agent: adding an organic decomposing agent to the animal manure whose water content has been adjusted and mixing, the amount of the organic decomposing agent added is 0.1wt% of the animal manure whose water content has been adjusted;

[0094] (3) High-temperature sterilization: use the heat of reaction between the organic decomposing agent and the animal manure to maintain the temperature...

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Abstract

The invention relates to the technical field of microorganisms, particularly to a bacillus amyloliquefaciens B6 and application thereof. The bacillus amyloliquefaciens B6 is preserved in CCTCC (china center for type culture collection), the preservation date is November 20, 2016, and the preservation No. is CCTCCM 2016621. The bacillus amyloliquefaciens B6 can inhibit 73 pathogenic bacteria of watermelons, is comparatively good in broad spectrum inhibition effect and high in endurance capacity and reproductive capacity, can be directly inoculated in a traditional planting site and still has a comparatively good effect even if no greenhouse is arranged.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus amyloliquefaciens B6 and an application thereof. Background technique [0002] In the prior art, soil-borne diseases refer to diseases caused by pathogens such as fungi, bacteria, nematodes and viruses that live in the soil along with diseased residues and attack crops from roots or stems when conditions are suitable. Continuous cropping is the main human factor for the formation of diseased soil. The main reason is that the continuous planting of a class of crops enables the corresponding certain pathogens to reproduce year after year, accumulate in a large amount in the soil, form diseased soil, and cause disease every year. [0003] Take watermelons as an example. Ningxia has 800,000 mu of sand-pressed watermelons. If the area of ​​watermelons and melons in Gansu and Xinjiang is included, it will reach nearly 10 million mu. These areas are traditional plantin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G31/00C12R1/07
CPCA01G31/00A01N63/00C12N1/205C12R2001/07
Inventor 司海丽张琇贾向峰王静林勤刘雅琴李敏张艳丽王翰霖杨晨希王宏雷王明杨国平
Owner BEIFANG UNIV OF NATITIES
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