A kind of seed production method of all female turbot zw seedlings

Abstract

The invention discloses a preparation method for scophthalmus maximus ZW all-female fry, and belongs to the technical field of fish cultivation. The method comprises the following steps: (1) a method of inducing mitotic gynogenesis through genetically-inactivated fish heterologous sperms is employed, so that scophthalmus maximus mitotic gynogenesis eggs are obtained; (2) the obtained mitotic gynogenesis eggs are subjected to brooding and obtained fry are cultivated, so that mitotic gynogenesis fry are obtained; (3) the obtained mitotic gynogenesis fry are bred to obtain mitotic gynogenesis reserve parent fish; (4) the mitotic gynogenesis reserve parent fish is subjected to maturity-promoting cultivation and egg-production regulation and control, and super-female parent fish is chosen; (5) scophthalmus maximus WW super-female parent fish and common ZZ male fish are subjected to artificial insemination, so that zygotes of which the genotypes are all ZW are obtained; and (6) the ZW zygotes are subjected to brooding and obtained fry are cultivated, so that the large-scale scophthalmus maximus all-female fry are obtained. The method proves that the scophthalmus maximus WW super-female fish can survive and be bred, and fertilization of the super-female parent fish and the common male fish is utilized to realize large-scale production of the scophthalmus maximus all-female fry, the fry preparation period is greatly shortened, and preparation costs are reduced.

Description

technical field [0001] The invention belongs to the technical field of fish breeding, and in particular relates to a seed production method of turbot ZW all female seedlings. Background technique [0002] Turbot (Scophthalmus maximus) is an excellent species of flounder flounder in the world, and its breeding range covers more than a dozen coastal countries in Europe, the United States and Asia. Among them, China's annual breeding production reaches 60,000 tons, accounting for 80% of the world's breeding production. The annual demand for flounder species is about 120 million fish (FEAP, 2016; National Flounder Industry Technology System Annual Report, 2015). Similar to flounder such as flounder and half-smooth tongue sole, there is a significant difference in growth rate between male and female turbot, and the body weight of female turbot is 1.8 times that of male after 2 years of full-sibling rearing in the same tank (Imsland et al., 1997, Aquaculture Research ,28:101–114)...

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Problems solved by technology

Martínez et al. 2014 (Front Genet.2014; 5:340.doi:10.3389 / fgene.2014.00340) and MariBaoiller&D'Cotta 2016 (Sex Dev 10:242-266, 2016) proposed a combination of hormone induction and test cross selection The method to prepare turbot super broodstock, and then use the super female fish to mate with ordinary male fish to produce all-female seedlings on a large scale, has not yet been fully implemented because of the long seed production cycle and high cost.

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