Screening method for coix drought resistance indexes

A screening method and index technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of confusing screening methods and inconsistent screening indexes, and achieve the effect of standardizing the drought resistance index screening technology system

Inactive Publication Date: 2017-11-07
贵州省旱粮研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a screening method for the drought resistance index of Job’s tears in view of the confusion and inconsistency of the curre

Method used

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  • Screening method for coix drought resistance indexes
  • Screening method for coix drought resistance indexes
  • Screening method for coix drought resistance indexes

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for screening drought-resistant indicators of Job's tears, comprising the following steps:

[0033] 1. Using 50 Coix germplasms as materials, select 50 uniform and plump seeds from each Coix germplasm, and use 0.1% HgCl 2 Rinse with distilled water for 5 times after disinfection for 8 minutes, soak the seeds in -0.1MPa PEG-6000 aqueous solution for 24 hours, then place them in a germination box with 24cm×24cm×12cm and covered with 3 layers of filter paper, the distance between seeds is 1.5 times of the grain length , add 15mL-0.1MPa PEG-6000 aqueous solution into the germination box, put the germination box in the RXZ-1000B artificial climate box, set the day / night temperature to 28°C / 25°C, humidity to 85%, and light / darkness to 12h / 12h, the light intensity is 340µmol / (m 2 ∙s), continuously cultured for 10 days, supplemented with appropriate amount of distilled water every 2 days to keep the osmotic potential unchanged, and performed the same treatment with di...

Embodiment 2

[0049] A method for screening drought-resistant indicators of Job's tears, comprising the following steps:

[0050] 1. Using 50 Coix germplasms as materials, pot experiments were carried out in dry sheds. Porcelain pots with a height of 50 cm and an inner diameter of 50 cm were filled with 20 cm-thick medium-fertility soil, and the total nutrients were applied to each pot before sowing. ≥45% high-efficiency compound fertilizer 10g, the content of high-efficiency compound fertilizer is 14%N, 16%P 2 o 5 , 15%K 2 0, and watered to 80% of the water holding capacity in the field, select 50 uniform and full seeds from each coix germplasm, disinfect with 0.1% HgCl for 8 minutes, rinse 5 times with distilled water, and dry them with filter paper Seeds were soaked in distilled water for 24 hours, and then 50 seeds per pot were sown. After sowing, the soil was covered with 2 cm. A random block design was adopted, and two treatments of normal water supply and repeated drought were set,...

Embodiment 3

[0064] A method for screening drought-resistant indicators of Job's tears, comprising the following steps:

[0065] 1. With 50 Coix germplasms as materials, field experiments were carried out in dry sheds, with two treatments of normal irrigation and drought stress, repeated three times, and the test materials for each treatment were arranged in random blocks, with an area of ​​10m 2 , 4m long, 2.5m wide, row spacing 50cm, litter spacing 20cm, 2 seedlings per litter, planting 5 rows, leaving a blank row between plots, block spacing 60 cm, sowing rows perpendicular to the block direction, around the test site Sow 3 rows for protection, and apply 300kg / hm of high-efficiency compound fertilizer with total nutrient ≥ 45% before sowing 2 As a seed fertilizer, the high-efficiency compound fertilizer content is 14%N, 16%P 2 o 5 , 15%K 2 O, the drought stress treatment is irrigated once before sowing to ensure that the seedlings are full, and no longer irrigate to the mature stage ...

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Abstract

The invention discloses a screening method for coix drought resistance indexes, and belongs to the technical field of agricultural production. The method includes the steps of (1) germplasm cultivation and planting of test coix; (2) determination of the relative drought resistance indexes; (3) principal component analysis; (4) gradual regression analysis. The screening method is close to the practice of agricultural production, has reliable results, and can solve the problems that the existing screening method for the coix drought resistance indexes is confused and the screened indexes are inconsistent, thereby providing a reliable, simple, economical, applicable and standard drought resistance index screening technology system for coix breeding workers; meanwhile, the screened indexes can comprehensively reflect the comprehensive drought resistance of the coix, and can be effectively applied to the identification of drought resistance of the coix.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to a method for screening drought-resistant indexes of coix coix. Background technique [0002] Coix coix is ​​used as both medicine and food. It has the functions of invigorating the spleen and dampness, relaxing muscles and eliminating numbness, clearing away heat and expulsion of pus, beautifying the skin, diuresis and reducing swelling, and preventing cancer. Its nutrition can be called "the king of grasses". Job's tears is a characteristic industry in Guizhou, among which Xingren County is the distribution center for the processing and sales of Job's tears in the whole country and even in Southeast Asia. With the warming of the global climate and the destruction of the ecological balance, drought has become the main restrictive factor for the agricultural production in my country and even the world's vast grain-producing areas for a long time. Guizhou Province ...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/00A01G7/06
Inventor 周棱波汪灿邵明波张国兵张立异徐燕高旭姜讷
Owner 贵州省旱粮研究所
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