Unlock instant, AI-driven research and patent intelligence for your innovation.

Bacterial strain, microbial agent and applications thereof

A technology of microbial agent and strain, which is applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems that straw rapid decomposition technology has not been effectively solved and limits the efficient use of straw resources, etc., to increase soil humus, improve The effect of soil fertility

Active Publication Date: 2017-11-07
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Straw rapid decomposition technology has not been effectively solved, which limits the efficient utilization of straw resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain, microbial agent and applications thereof
  • Bacterial strain, microbial agent and applications thereof
  • Bacterial strain, microbial agent and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This embodiment provides a screening method for microbial strains and a combination method for bacterial agents.

[0049] 1. Experimental materials

[0050] 1.1 Strains and soil

[0051] Tested strains: Cellulose-degrading bacteria preserved in the strain bank.

[0052] Northeast black soil: 0-20cm topsoil in Gongzhuling field, Jilin.

[0053] 1.2 Medium

[0054] 1.2.1 Bacterial medium: beef extract 3g, peptone 10g, NaCl 5g, agar 16-18g, deionized water 1L.

[0055] 1.2.2 Bacterial fermentation medium (YPD complete nutrient medium): dissolve yeast extract / yeast powder 10g / L, peptone 20g / L in 1L deionized water, pH 7.0.

[0056] 1.2.3 Culture medium for cellulose-degrading bacteria: KH 2 PO 4 0.5g, MgSO 4 0.25g, peptone 2g, sodium carboxymethylcellulose (CMC) 1.88g, Congo red 0.2g, agar 16-18g, deionized water 1L, pH 7.0.

[0057] 1.2.4 Basic nutrient solution (Hutchison's medium): potassium dihydrogen phosphate 1.0g, NaCl 0.1g, NaNO 3 2.5g, 1L deionized water,...

Embodiment 2

[0079] This embodiment provides a liquid bacterial agent and a preparation method thereof, specifically comprising the following steps:

[0080] Prepare a single bacterial liquid in the bacterial fermentation medium (1.2.2), and the bran powder absorbs the single bacterial liquid according to the solid-liquid ratio of 1:2 to prepare single bacterial agents A1, A2, B2, D3; 4 kinds of single bacterial agents Mix in equal proportions, air-dry and pack into bags to complete the preparation of the composite bacterial agent.

[0081] The content of each bacterial strain in the obtained liquid bacterial agent was measured to be 1×10 10-11 CFU / ml.

Embodiment 3

[0083] In the present embodiment, the preparation operation is the same as in Example 1, and the difference from Example 1 is that in the present embodiment, the single bacteria agents A1, B2, and D3 are prepared; 3 kinds of single bacteria agents are according to the number of live bacteria 1:3: 5 ratio, air-dried and bagged to complete the preparation of the composite bacterial agent.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microbe application, and particularly relates to a bacterial strain, a microbial agent and applications thereof. The strain is bacillus flexus A1, which is preserved in China General Microbiological Culture Collection Center at May 4th, 2017, with number of CGMCC NO.14088. The microbial agent containing the bacterial strain is especially suitable for rapid degradation of north maize straws, has important function in the in-situ returning to the field of crop straws, and also has the functions of being capable of increasing soil humus and improving soil fertility.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a bacterial strain, a microbial agent and an application thereof. Background technique [0002] my country is rich in crop straw resources, with a huge quantity and various types. The annual output of crop straw is 700-800 million tons. Because the straw is not easy to decompose in a short period of time, and the utilization efficiency is low, most of the straw is discarded or burned. In areas where planting is the mainstay, crop straw has become an obstacle in production. Therefore, straw burning spreads all over the vast countryside, which causes environmental pollution and serious waste of organic resources. According to statistics, the crop straw used for returning to fields and organic fertilizers in the country only accounts for about 20% of the total, incineration accounts for 17%, and 50% remains unused. [0003] Many provinces in northern my country are l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C05F11/08C12R1/07
CPCC12N1/20C05F11/08C12R2001/07C12N1/205Y02W30/40
Inventor 任萍顾金刚卢昌艾
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI