Rape species identification method based on terahertz spectroscopy

Abstract

The invention discloses a rape species identification method based on terahertz spectroscopy. The method comprises the steps that rape leaf samples are selected from two different species of rapes respectively, a terahertz time-domain spectrometer is adopted for collecting reference signals and terahertz time-domain spectra of rape leaves; the terahertz time-domain spectra are converted into terahertz frequency spectra; a transmittance spectrum, a refractive index spectrum, an absorption coefficient spectrum and an extinction coefficient spectrum are extracted from the terahertz frequency spectra; four optical parameter spectra, subjected to spectrum processing, of two species of the rapes are subjected to classification and identification, and rape species identification based on the terahertz spectroscopy is achieved. Accordingly, by means of the terahertz spectroscopy, differentiation and identification situations of different species of the rapes are researched, certain theoretical and practical guiding significance is achieved for establishment of a technology platform of rape species rapid identification, optimal screening of rape species and seed breeding fine management, and an application prospect of THz in the field of agriculture and agricultural product species identification is shown.

Description

technical field [0001] The invention relates to a method for distinguishing varieties of rapeseed, in particular to a method for identifying varieties of rapeseed based on terahertz spectrum technology. Background technique [0002] Rapeseed is an agronomic collective term for several crops of the Brassica genus that can produce oil-pressed seeds. Rapeseed is one of the most important raw materials and economic crops for oil production in my country, accounting for more than 40% of the total area of ​​oil economic crops in my country and more than 30% of the total oil production. Rapeseed is the main crop for oil production. Rapeseed oil is rich in unsaturated fatty acids such as oleic acid and linoleic acid, which has health benefits such as preventing cardiovascular diseases and reducing serum cholesterol in the human body. In my country, there are about 15 different varieties of rapeseed. Different varieties of rapeseed have certain morphological characteristics, yield t...

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Problems solved by technology

[0003] For the identification of rapeseed varieties, the PAGE silver staining method is generally used at present, but this detection method has disadvantages such as low detection efficiency, poor repeatability, and inability to accurately identify fragment sizes; in addition, there is SSR fluorescent labeling capillary electrophoresis detection technology, but this This technology is DNA-based fingerprint identification, which is complex, time-consuming and labor-intensive; this type of detection method requires a lot of time and energy in sample preparation, and some waste liquids and wastes cause serious pollution to the environment, and destructive experiments will The plant growth process cannot continue, and the long detection time cannot guarantee the timeliness of the data and the synchronization between the measurement data of different samples

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