A transport preservation solution and preparation method for maintaining the activity of mesenchymal stem cells
A technique for mesenchymal stem cells and preservation solution, which is applied in the field of preservation solution for maintaining the activity of mesenchymal stem cells and its preparation, can solve the problems of affecting the therapeutic effect of MSCs, limiting the application scope of MSCs, and limiting the effect, and achieves the expansion of the geographical scope of clinical use, The effect of maintaining vitality and good protection
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Embodiment 1
[0027] Example 1 The protective effect of compound amino acids on mesenchymal stem cells
[0028] In order to compare the protective effects of compound amino acids and glucose on the viability of mesenchymal stem cells, the mesenchymal stem cell transport preservation solution was divided into 3 groups of different formulations in this example.
[0029] Group 1: without cell viability protection agent: Mix 75ml of compound electrolyte injection, 25ml of stock solution concentration of 20% human albumin (final concentration of 0.05g / ml).
[0030] Group 2: only include glucose: 72ml of compound electrolyte injection, 20% human serum albumin 25ml (final concentration of 0.05g / ml) and 3ml of glucose injection (final concentration of 0.05g / ml) with stock solution concentration 1.5mg / ml) and mix well.
[0031] Group 3: only containing compound amino acids: compound amino acid injection (18AA) with 74ml of compound electrolyte injection, 25ml of human serum albumin (final concentra...
Embodiment 2
[0037] Example 2 The protective effect of melatonin and rapamycin on mesenchymal stem cells
[0038] In order to further optimize the formulation of the transport preservation solution, in this example, on the basis of adding compound amino acids, the cell viability protective agents melatonin and rapamycin were added, and the effects of melatonin and rapamycin on mesenchymal stem cells were determined. Vitality protection. In this example, the mesenchymal stem cell transport preservation solution was divided into 3 groups.
[0039] Group 4: Containing compound amino acid and melatonin: compound amino acid injection with 73ml compound electrolyte injection, 20% stock solution concentration of human albumin 25ml (final concentration 0.05g / ml), stock solution concentration 0.09g / ml (18AA) 1ml (final concentration: 0.9mg / ml), stock solution concentration: 0.115mg / ml melatonin solution 1ml (final concentration: 1.15μg / ml) and mix well.
[0040] Group 5: Containing compound amino...
Embodiment 3
[0048] Example 3 The protective effect of different preservation solution formulations on mesenchymal stem cells
[0049] In order to compare the transport preservation solution in the present invention with the mesenchymal stem cell preservation solution and injection solution in other patents, this embodiment selected two reported formulas and the formula of the present invention (Example 2, Group 6) for cell viability Determination test.
[0050] Patent A formula: normal saline, human serum albumin (5%), low molecular weight heparin calcium (75AXaIU / mL)
[0051] Patent B formula: normal saline, autologous platelet lysate (4%), vitamin C injection (1%)
[0052] Formula of the present invention: compound electrolyte injection, human serum albumin (5%), compound amino acid injection (0.9mg / ml), melatonin (1.15 μ g / mL), rapamycin (100nM)
[0053] The transport solution of the above-mentioned groups of formulas was filtered and sterilized with a filter with a pore size of 0.2 ...
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