Application of integrated COP nanosheet arrays as monolithic peroxidase-like enzymes

A peroxidase and nanosheet array technology, applied in the field of simulated enzymes, can solve the problems of cumbersome recycling process and difficult recycling, achieve convenient recycling and reuse, excellent recycling effect, increase surface area and utilization rate Effect

Active Publication Date: 2020-01-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Although the above-mentioned disclosed peroxidase-like enzymes have high catalytic activity and fast reaction rate, they have the following common disadvantages: these peroxidase-like enzymes all act by dissolving or dispersing in the aqueous phase solution, and most of them are difficult to recycle. Can be recycled, but the recycling process needs to be centrifuged or separated by an external magnetic field, and the recycling process is cumbersome

Method used

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  • Application of integrated COP nanosheet arrays as monolithic peroxidase-like enzymes
  • Application of integrated COP nanosheet arrays as monolithic peroxidase-like enzymes
  • Application of integrated COP nanosheet arrays as monolithic peroxidase-like enzymes

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The preparation method of the integrated CoP nanosheet array as a monolithic peroxidase comprises the following steps:

[0040] 1) Cut the commercial nickel foam into a square of 1cm×1cm, soak it with 37% hydrochloric acid for 30 minutes and ultrasonically clean it with deionized water for 10 minutes, then move it to a 60°C constant temperature drying oven to dry to obtain pure foam nickel;

[0041] 2) Dissolve 1.4055g of cobalt sulfate and 0.3g of urea into 30mL of deionized water, and mix well by stirring at room temperature for 10min;

[0042] 3) Put the treated nickel foam (30 pieces) and the above solution in a hydrothermal reaction kettle for 12 hours at 80°C to obtain Co(OH) 2 Nanosheet array modified nickel foam composite;

[0043] 4) Rinse the above complex with deionized water, and dry it in an oven at 60°C. Dry Co(OH) 2 / NF tablets (6 pieces) and 300mg sodium hypophosphite were respectively placed in two adjacent porcelain boats (the porcelain boats were pla...

Embodiment 2

[0048] The preparation method of the integrated CoP nanosheet array as a monolithic peroxidase comprises the following steps:

[0049] 1) Cut the commercial nickel foam into a square of 1cm×1cm, soak it with 37% hydrochloric acid for 30 minutes and ultrasonically clean it with deionized water for 10 minutes, then move it to a 60°C constant temperature drying oven to dry to obtain pure foam nickel;

[0050] 2) Dissolve 1.4055g of cobalt sulfate and 0.9g of urea into 60mL of deionized water, and mix well by stirring at room temperature for 20min;

[0051] 3) Put the treated nickel foam (30 pieces) and the above solution in a hydrothermal reaction kettle for 24 hours at 150°C to obtain Co(OH) 2 Nanosheet array modified nickel foam composite;

[0052] 4) Rinse the above complex with deionized water, and dry it in an oven at 60°C. Dry Co(OH) 2 / NF tablets (6 pieces) and 450mg sodium hypophosphite were put into two adjacent porcelain boats respectively (the porcelain boats were p...

Embodiment 3

[0057] The preparation method of the integrated CoP nanosheet array as a monolithic peroxidase comprises the following steps:

[0058] 1) Cut the commercial nickel foam into a square of 1cm×1cm, soak it with 37% hydrochloric acid for 30 minutes and ultrasonically clean it with deionized water for 10 minutes, then move it to a 60°C constant temperature drying oven to dry to obtain pure foam nickel;

[0059] 2) Dissolve 1.4055g of cobalt sulfate and 1.9g of urea into 100mL of deionized water, and mix well by stirring at room temperature for 20min;

[0060] 3) Put the treated foamed nickel (30 pieces) and the above solution in a hydrothermal reaction kettle for 48 hours at 120°C to obtain Co(OH) 2 Nanosheet array modified nickel foam composite;

[0061] 4) Rinse the above complex with deionized water, and dry it in an oven at 60°C. Dry Co(OH) 2 / NF tablets (6 pieces) and 600mg sodium hypophosphite were put into two adjacent porcelain boats respectively (the porcelain boats wer...

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Abstract

The invention belongs to the technical field of analogue enzymes, and relates to preparation of an analogue peroxidise, in particular to preparation and application of an integrated CoP nano-flake array serving as an integrated analogue peroxidise. A preparation method for the integrated CoP nano-flake array serving as the integrated analogue peroxidise comprises the following steps: performing pre-treatment to obtain pure nickel foam, then dissolving cobalt sulphate and urea as a solution, adding the nickel foam into the solution, performing a hydrothermal reaction to obtain a Co(OH)2 nano-flake array-modified foam nickel compound, and performing high-temperature phosphating to obtain the integrated CoP / NF integrated analogue peroxidise. The invention also discloses the application of the product to visual detection of hydrogen peroxide. According to the preparation method, the integrated analogue peroxidise is prepared by utilizing porous nickel foam as a supporting body, the surface area and the utilization rate of an analogue enzyme are effectively improved, a reaction process can be controlled, on-demand taking is realized, and a quenching agent is not required. The integrated analogue peroxidise can also be recycled and reused conveniently, and the integrated analogue peroxidise has an excellent reutilization effect.

Description

technical field [0001] The invention belongs to the technical field of simulated enzymes, relates to the application of peroxidase-like enzymes, in particular to the application of an integrated CoP nanosheet array as an integral peroxidase-like enzyme. Background technique [0002] Natural enzymes can catalyze a series of reactions with high efficiency and high selectivity under mild conditions, and are widely used as biocatalysts in the fields of pharmacy, medicine and analysis and detection. However, natural enzymes are easily denatured and inactivated by environmental factors such as pH, temperature, and chemical reagents during use. Moreover, the extraction and purification process is time-consuming and labor-intensive, resulting in high price, and it is difficult to recycle after use, which seriously limits its practical application in many aspects. [0003] Since Yan Xiyun's research group discovered that Fe3O4 nanoparticles have peroxidase-like catalytic properties ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J27/185G01N21/31
CPCB01J27/1853G01N21/31
Inventor 何艳芳牛湘衡张文驰齐飞宋洪伟张锡凤
Owner JIANGSU UNIV
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