Experimental method of lycium barbarum polysaccharide in inhibition of glioma growth by regulating blood brain barrier

A technology of Lycium barbarum polysaccharide and blood-brain barrier, which is applied in biochemical equipment and methods, microbial determination/inspection, biological testing, etc., can solve problems such as little effect of drugs, and achieves lightening of family burden, extensive planting, and low price. Effect

Inactive Publication Date: 2017-11-24
沈冰
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Problems solved by technology

[0004] The purpose of the present invention is to provide a technical scheme of an experimental method in which Lycium barbarum polysaccharide inhibits the growth of glioma by regulating the blood-brain barrier in view of the shortcomings of the existing situation that the blood-brain barrier has little drug effect

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  • Experimental method of lycium barbarum polysaccharide in inhibition of glioma growth by regulating blood brain barrier
  • Experimental method of lycium barbarum polysaccharide in inhibition of glioma growth by regulating blood brain barrier
  • Experimental method of lycium barbarum polysaccharide in inhibition of glioma growth by regulating blood brain barrier

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Embodiment Construction

[0021] The specific embodiment of the present invention is as follows.

[0022] 1. Grouping and feeding of experimental animals: ① 200 male SD rats with standard weight (180-200g) were randomly divided into 6 groups, 25 rats in each group (7 rats were observed for survival, 5 rats were injected with Evans blue for blood test). Changes in the brain barrier, 7 brains perfused with 4% paraformaldehyde to measure tumor size and perform immunohistochemical experiments, 6 decapitated brains were used for RT-PCR and Western blotting experiments), rats in each group were randomly assigned: 4 -5 rats / cage; extra rats were prepared in each group to prevent the failure of modeling or other accidental factors from affecting the experiment; ②The drinking water of the rats in the blank control group was tap water throughout the whole process, and the drinking water of the rats in the experimental group was of different concentrations Lycium barbarum polysaccharide solution (3 days before th...

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Abstract

The invention provides an experimental method of lycium barbarum polysaccharide in inhibition of glioma growth by regulating a blood brain barrier and belongs to the field of traditional Chinese medicine. The experimental method comprises the following steps: taking a male SD rat, feeding for 3-4 weeks with lycium barbarum polysaccharide solution; then constructing a rat glioma model; molding, continuing feeding with the lycium barbarum polysaccharide solution, and detecting, namely firstly detecting survival time, survival rate and median survival time of rats in each group; secondly taking brain tissues of the rats in each group, observing and measuring tumor size; thirdly judging permeability change of the blood brain barrier by carrying out femoral vein Evans blue solution on the rats in each group, taking the same amount of brain tissues and detecting Evans blue content; fourthly observing ultrastructure change of the blood brain barrier by virtue of an electron microscope; fifthly carrying out cardiac apex injection on the rats in each group with paraformaldehyde solution, taking the brain tissues, slicing and then carrying out an immunohistochemical test; and sixthly directly cutting off heads of the rats in each group, taking brains, extracting RNA and proteins and carrying out RT-PCR and Western blotting experiments. The experimental method provided by the invention provides a new treatment approach for treatment of multiple blood brain barrier diseases.

Description

technical field [0001] The invention belongs to the field of application of traditional Chinese medicine, and in particular relates to an experimental method for inhibiting the growth of glioma by regulating the blood-brain barrier with Lycium barbarum polysaccharide. Background technique [0002] Glioblastoma is the most common malignant brain tumor in adults. Its most typical feature is a high degree of infiltration into the surrounding brain tissue. Since the infiltrating tumor cells cannot be completely removed, new tumor tissue recurs and forms, resulting in a very poor prognosis for patients. , despite aggressive surgery, radiotherapy, chemotherapy, and supplemented with new molecular biology therapies, the median survival period of patients was only extended to 2 years. Molecular diagnosis shows that the pathological morphology of glioblastoma is diverse and variable, which makes the treatment more difficult. Current studies have shown that the antineoplastic drug te...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/577
CPCC12Q1/686G01N33/577G01N33/68C12Q2521/107
Inventor 沈冰王军成邹有瑞
Owner 沈冰
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