Method of detecting cadmium in water sample based on orthophenanthroline-carmine fluorescence method

Abstract

The invention belongs to the technical field of environmental pollution detection and discloses a method of detecting cadmium in a water sample based on an orthophenanthroline-carmine fluorescence method. The method comprises the following steps: adding 0.50mL of citric acid-disodium hydrogen phosphate buffer solution with the pH of 4.5 and 1.00ml of 0.30*10<-4>mol / L orthophenanthroline as well as a 0.60ml of 0.12*10<-4>mol / L carmine solution; performing a reaction for 15min, stabilizing the reaction for 6 h or more than 6h, and measuring the fluorescence intensity value 30min after the reaction; and by taking deltaF as a vertical coordinate and cadmium concentration as a transverse coordinate, drawing a standard curve to solve the concentration of the water sample. The instrument is low in price; the detection threshold is low; the sample detection cost is low; and the operating steps are simple and the reaction is quick.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution detection, and in particular relates to a method for detecting cadmium in water samples based on the o-phenanthroline-carmine fluorescence method. Background technique [0002] Cadmium (Cd) not only seriously pollutes the environment, but also irreversibly damages the health of humans, animals and plants. In industrial environments, such as electroplating factories, dye factories, metallurgical and chemical factories, etc., there are often cadmium and its compound pollutants in the wastewater and waste residues, which mainly enter the human body through the respiratory and digestive tracts; then accumulate in the body and affect human immunity. , reproductive system, kidney, bone, and liver produce toxic effects, leading to a series of diseases in the human body. In an environment with high cadmium content, it can cause human poisoning, nausea, vomiting, and diarrhea in mild cases...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problem existing in the existing technology is: the instruments used are relatively expensive, the price ranges from two hundred thousand to several million, and it is difficult to popularize them in ordinary laboratories.

The detection cost is relatively high, and the market price is basically 200-400 yuan / sample. Due to complex matrix interference, large errors in the sampling system of instruments and equipment, etc., there are many influencing factors and the accuracy is not high.

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