Test strip for detecting low-density lipoprotein cholesterol in serum, and preparation method thereof
A low-density lipoprotein and test strip technology, which is applied in the field of test strips for detecting low-density lipoprotein cholesterol in serum and its preparation, can solve the problems of many experimental steps, cumbersome preparation methods, and complicated operations
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Embodiment 1
[0045] (1) Prepare total cholesterol (TC) detection reagent: cholesterol lipase (CHER) 5KU / L, cholesterol oxidase (CHOD) 40KU / L, MgSO 4 280mM, horseradish peroxidase 150KU / L, 4-aminoantipyrine 18 mM, N-ethyl-N-(2-hydroxy-3-propanesulfo)m-toluidine (TOOS) 45 mM concentration , using phosphoric acid (PBS) as the buffer, add all the above reagents into a 15mL EP tube and mix well.
[0046] ) In the reagent obtained in the first step, add 0.14 g of Tween-20 (Tween-20) to 1 mL of reagent, and then add Poloxamer F124 with a number average molecular weight of 8400 at a concentration of 1.8 g / L, and mix well.
[0047] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips with a width of about 0.5cm, put it into the solution prepared in the previous step, and soak it for 1.5 hours. Then take it out, put it into an electric heating blast drying box, and dry it at 45 degrees Celsius for 60 minutes. The fully dried membrane strip is the LDL-c dry chemical reaction she...
Embodiment 2
[0052] (1) Prepare total cholesterol (TC) detection reagent: cholesterol lipase (CHER) 15KU / L, cholesterol oxidase (CHOD) 60KU / L, MgSO 4 320mM, horseradish peroxidase 250KU / L, 4-aminoantipyrine 22 mM, N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) 55 mM Concentration, using phosphoric acid (PBS) as the buffer, add all the above reagents into a 15mL EP tube and mix well.
[0053] ) In the reagent obtained in the first step, add 0.18g Tween-20 (Tween-20) to 1mL of reagent, then add Poloxamer F88 with a number average molecular weight of 8400 at a concentration of 2.2g / L, and mix well.
[0054] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips with a width of about 0.5cm, put it into the solution prepared in the previous step, and soak it for 1.5 hours. Then take it out, put it into an electric heating blast drying box, and dry it at 45 degrees Celsius for 60 minutes. The fully dried membrane strip is the LDL-c dry chemical reaction sheet. ...
Embodiment 3
[0059] (1) Preparation of total cholesterol (TC) detection reagent: cholesterol lipase (CHER) 10KU / L, cholesterol oxidase (CHOD) 50KU / L, MgCl 2 300 mM, horseradish peroxidase 200 KU / L, 4-aminoantipyrine 20 mM, nitrotetrazolium chloride (NBT) 50 mM, phosphoric acid (PBS) as a buffer, the above reagents were mixed All were added to a 15mL EP tube and mixed well.
[0060] ) In the reagent obtained in the first step, add 0.16g NP-10 to 1mL of reagent, then add Poloxamer F88 with a number average molecular weight of 6000 at a concentration of 2g / L, and mix well.
[0061] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips with a width of about 0.5cm, put it into the solution prepared in the previous step, and soak it for 1.5 hours. Then take it out, put it into an electric heating blast drying box, and dry it at 45 degrees Celsius for 60 minutes. The fully dried membrane strip is the LDL-c dry chemical reaction sheet.
[0062] ) Using a 2 mm thick glass fibe...
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