Test strip for detecting low-density lipoprotein cholesterol in serum, and preparation method thereof

A low-density lipoprotein and test strip technology, which is applied in the field of test strips for detecting low-density lipoprotein cholesterol in serum and its preparation, can solve the problems of many experimental steps, cumbersome preparation methods, and complicated operations

Active Publication Date: 2017-11-28
LUMIGENEX (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, when detecting the LDL-c concentration in the above-mentioned technology, there are the following problems: the scheme of separating LDL and HDL by ultracentrifugation needs to use an expensive ultrahigh-speed centrifuge as the main separation equipment, and there are many experimental steps and the operation is very complicated , need to prepare a variety of reagents, the most important thing is very time-consuming, the whole separation time is often more than 6 hours
It can be seen that the dry analysis element made by this patent requires two kinds of reagents to be fixed on different films respectively, so the preparation method is cumbersome

Method used

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  • Test strip for detecting low-density lipoprotein cholesterol in serum, and preparation method thereof
  • Test strip for detecting low-density lipoprotein cholesterol in serum, and preparation method thereof
  • Test strip for detecting low-density lipoprotein cholesterol in serum, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Prepare total cholesterol (TC) detection reagent: cholesterol lipase (CHER) 5KU / L, cholesterol oxidase (CHOD) 40KU / L, MgSO 4 280mM, horseradish peroxidase 150KU / L, 4-aminoantipyrine 18 mM, N-ethyl-N-(2-hydroxy-3-propanesulfo)m-toluidine (TOOS) 45 mM concentration , using phosphoric acid (PBS) as the buffer, add all the above reagents into a 15mL EP tube and mix well.

[0046] ) In the reagent obtained in the first step, add 0.14 g of Tween-20 (Tween-20) to 1 mL of reagent, and then add Poloxamer F124 with a number average molecular weight of 8400 at a concentration of 1.8 g / L, and mix well.

[0047] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips with a width of about 0.5cm, put it into the solution prepared in the previous step, and soak it for 1.5 hours. Then take it out, put it into an electric heating blast drying box, and dry it at 45 degrees Celsius for 60 minutes. The fully dried membrane strip is the LDL-c dry chemical reaction she...

Embodiment 2

[0052] (1) Prepare total cholesterol (TC) detection reagent: cholesterol lipase (CHER) 15KU / L, cholesterol oxidase (CHOD) 60KU / L, MgSO 4 320mM, horseradish peroxidase 250KU / L, 4-aminoantipyrine 22 mM, N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) 55 mM Concentration, using phosphoric acid (PBS) as the buffer, add all the above reagents into a 15mL EP tube and mix well.

[0053] ) In the reagent obtained in the first step, add 0.18g Tween-20 (Tween-20) to 1mL of reagent, then add Poloxamer F88 with a number average molecular weight of 8400 at a concentration of 2.2g / L, and mix well.

[0054] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips with a width of about 0.5cm, put it into the solution prepared in the previous step, and soak it for 1.5 hours. Then take it out, put it into an electric heating blast drying box, and dry it at 45 degrees Celsius for 60 minutes. The fully dried membrane strip is the LDL-c dry chemical reaction sheet. ...

Embodiment 3

[0059] (1) Preparation of total cholesterol (TC) detection reagent: cholesterol lipase (CHER) 10KU / L, cholesterol oxidase (CHOD) 50KU / L, MgCl 2 300 mM, horseradish peroxidase 200 KU / L, 4-aminoantipyrine 20 mM, nitrotetrazolium chloride (NBT) 50 mM, phosphoric acid (PBS) as a buffer, the above reagents were mixed All were added to a 15mL EP tube and mixed well.

[0060] ) In the reagent obtained in the first step, add 0.16g NP-10 to 1mL of reagent, then add Poloxamer F88 with a number average molecular weight of 6000 at a concentration of 2g / L, and mix well.

[0061] ) Cut the nitrocellulose membrane with a pore size of 0.4um into strips with a width of about 0.5cm, put it into the solution prepared in the previous step, and soak it for 1.5 hours. Then take it out, put it into an electric heating blast drying box, and dry it at 45 degrees Celsius for 60 minutes. The fully dried membrane strip is the LDL-c dry chemical reaction sheet.

[0062] ) Using a 2 mm thick glass fibe...

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Abstract

The invention relates to a test strip for detecting low-density lipoprotein cholesterol in serum. The test strip includes a dry chemical reaction chip, the dry chemical reaction chip is prepared by soaking a reagent pad in a chemical reagent and then drying the reagent pad, and the chemical reagent is prepared from cholesterol esterase, cholesterol oxidase, peroxidase, metal salt, a color developer, polyoxyethylene ether and an ethylene oxide-propylene oxide copolymer having preferable concentrations according to a preferable ratio. The test strip has the advantages of low cost, easiness in preparation and obtaining, quickness in reaction and convenience in operation.

Description

technical field [0001] The invention relates to a test strip for detecting low-density lipoprotein cholesterol in serum and a preparation method thereof. Background technique [0002] Abnormal lipid metabolism is one of the most important risk factors for coronary heart disease (CHD). Many large-scale clinical studies have fully demonstrated the important role of cholesterol, especially low-density lipoprotein cholesterol (LDL-C), in the occurrence and development of coronary heart disease. Reducing LDL-C can significantly reduce coronary heart disease events. Elevated low-density lipoprotein cholesterol is positively correlated with the incidence of coronary heart disease, and is one of the important analytical indicators of coronary heart disease. Conventional LDL-C concentrations are obtained by separate measurements or indirect approximations. Separation and determination is to use an ultracentrifuge to separate the lipoprotein components in blood samples into high-den...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 何爱民黄芳婷刘星
Owner LUMIGENEX (SUZHOU) CO LTD
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