Method for determining content of effective components of ginseng

A technology of active ingredients and analysis methods, applied in the field of content determination of active ingredients in ginseng, can solve problems affecting the accuracy, reproducibility and selectivity of content determination methods, inability to use matrix matching correction method, and inconsistencies, etc.

Inactive Publication Date: 2017-11-28
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above examples all show that the detection and content determination methods of traditional Chinese medicine components are relatively mature qualitative and quantitative analysis methods in the field of pharmaceutical analysis. Unpredictable interfering compounds coexist with analytes. The standard calibration curve obtained by using a series of concentration standard solutions diluted in pure solvents does not take into account the existence of matrix effects, ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining content of effective components of ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention specifically provides a method for determining the content of active ingredients of ginseng, comprising the following steps:

[0020] Using the standard superposition method to determine the four active ingredients under the "Pharmacopoeia of the People's Republic of China" ginseng content determination requirements, ginsenoside Rg 1 (1), Re (2), Rb 1 (3), Rd (4) for quantitative research. An evaporative light scattering detector is mainly used in this example. The specific implementation process of the present invention is illustrated through this example.

[0021] The chromatographic analysis of ginseng samples was performed using an Agilent 1100 liquid phase system (Agilent Technologies, PaloAlto, CA, USA), including an online vacuum degasser, a binary gradient pump, a disc autosampler, and a column oven. Evaporative light scattering detector (ELSD, Alltech Associates, Deerfield, USA), the drift tube temperature of ELSD was 106 °C, and the ca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention establishes a ginseng effective component content determination method-standard superposition method meeting the characteristics of traditional Chinese medicines, relating to the field of quality control of traditional Chinese medicines. According to the method, a ginseng extracting solution is used as a dilution solution for diluting a to-be-quantitatively-researched target effective component standard solution into a series of concentration standard operation solutions, the concentration standard operation solutions are detected by virtue of a detector, a standard correction curve of the target effective component is determined, and therefore, the content of the target effective component is determined; and problems that when a standard correction curve is obtained by diluting a series of concentration standard solutions by virtue of a pure solvent, the existence of a matrix effect is not considered, so that the detection environments of an analyte in the pure solvent and a traditional Chinese medicine extracting solution are quite different, and the accuracy, repeatability and selectivity of a content determination method are influenced are solved. By utilizing the standard superposition method, the detection environments of the target effective component in different detectors can be furthest reappeared, so that the interference of co-elution endogenous substances to the target effective component are eliminated, the influence of the matrix effect is effectively eliminated, and a content determination result of the ginseng effective component is relatively accurate.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a method suitable for determining the content of active ingredients in ginseng. In the method, the extract of ginseng itself is used as a dilution solution to dilute the standard solution of active ingredients required for quantitative research into a series of concentration standard working solutions Afterwards, it is detected by the detector to determine the standard calibration curve of the target analyte, so as to determine the content of the target active ingredient. Background technique [0002] Ginsenoside Rb1, Chinese chemical name: (3β, 12β)-20-[(6-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-12-hydroxydammarate-24 -ene-3-y12-O-β-D-glucopyranosyl, English chemical name: (3β, 12β)-20-[(6-O-β-D-Glucopyranosyl-β-D-glucopyranosyl)oxy] -12-hydroxydammar-24-en-3-yl2-O-β-D-glucopyranosyl-β-D-glucopyranoside. Ginsenoside Rb1 exists in the roots...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 刘颖
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products