Method for determining content of effective components of ginseng

Abstract

The invention establishes a ginseng effective component content determination method-standard superposition method meeting the characteristics of traditional Chinese medicines, relating to the field of quality control of traditional Chinese medicines. According to the method, a ginseng extracting solution is used as a dilution solution for diluting a to-be-quantitatively-researched target effective component standard solution into a series of concentration standard operation solutions, the concentration standard operation solutions are detected by virtue of a detector, a standard correction curve of the target effective component is determined, and therefore, the content of the target effective component is determined; and problems that when a standard correction curve is obtained by diluting a series of concentration standard solutions by virtue of a pure solvent, the existence of a matrix effect is not considered, so that the detection environments of an analyte in the pure solvent and a traditional Chinese medicine extracting solution are quite different, and the accuracy, repeatability and selectivity of a content determination method are influenced are solved. By utilizing the standard superposition method, the detection environments of the target effective component in different detectors can be furthest reappeared, so that the interference of co-elution endogenous substances to the target effective component are eliminated, the influence of the matrix effect is effectively eliminated, and a content determination result of the ginseng effective component is relatively accurate.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a method suitable for determining the content of active ingredients in ginseng. In the method, the extract of ginseng itself is used as a dilution solution to dilute the standard solution of active ingredients required for quantitative research into a series of concentration standard working solutions Afterwards, it is detected by the detector to determine the standard calibration curve of the target analyte, so as to determine the content of the target active ingredient. Background technique [0002] Ginsenoside Rb1, Chinese chemical name: (3β, 12β)-20-[(6-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-12-hydroxydammarate-24 -ene-3-y12-O-β-D-glucopyranosyl, English chemical name: (3β, 12β)-20-[(6-O-β-D-Glucopyranosyl-β-D-glucopyranosyl)oxy] -12-hydroxydammar-24-en-3-yl2-O-β-D-glucopyranosyl-β-D-glucopyranoside. Ginsenoside Rb1 exists in the roots...

AI Technical Summary

Problems solved by technology

The above examples all show that the detection and content determination methods of traditional Chinese medicine components are relatively mature qualitative and quantitative analysis methods in the field of pharmaceutical analysis. Unpredictable interfering compounds coexist with analytes. The standard calibration curve obtained by using a series of concentration standard solutions diluted in pure solvents does not take into account the existence of matrix effects, which makes the detection environment of analytes in pure solvents and traditional Chinese medicine extracts very different. , thus affecting the accuracy, reproducibility and selectivity of the assay method

At the same time, traditional Chinese medicine extracts are also different from biological fluids. It is difficult to obtain blank matrix samples without target analytes, so the matrix matching correction method for the determination of biological samples cannot be used.

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