Extraction method of sterol and sterol

An extraction method and sterol technology, which are applied to the sterol extraction method and the sterol field, can solve the problems of low yield, high cost, complicated purification process and the like, and achieve the effects of high product purity, low production cost and high application value.

Inactive Publication Date: 2017-12-01
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the existing extraction methods have the problems

Method used

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  • Extraction method of sterol and sterol
  • Extraction method of sterol and sterol

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Experimental program
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Effect test

Embodiment 1

[0043] The present embodiment provides a kind of extraction method of sterol, it comprises the following steps:

[0044]First, after mixing 50 mL of seabuckthorn seed oil with 400 mL of potassium hydroxide solution with a concentration of 0.5 mol / L, the saponification reaction was carried out at a temperature of 75° C. for 230 min to obtain a saponified liquid.

[0045] Next, take 400 mL of the above saponified solution, add water to dilute to 1000 mL, extract with 400 mL of petroleum ether, filter to obtain the extract, and evaporate the extract to dryness at a temperature of 27°C.

[0046] This embodiment also provides a sterol, which is prepared by the above-mentioned method.

Embodiment 2

[0048] The present embodiment provides a kind of extraction method of sterol, it comprises the following steps:

[0049] First, after mixing 50 mL of seabuckthorn seed oil with 400 mL of potassium hydroxide solution with a concentration of 0.5 mol / L, the saponification reaction was carried out at a temperature of 75° C. for 230 min to obtain a saponified liquid.

[0050] Next, take 400mL of the above saponification solution, add water to dilute to 1000mL, extract with 400mL of petroleum ether, filter to obtain the extract, evaporate the extract at a temperature of 27°C, and dry the evaporated residue to obtain a paste Initial product.

[0051] Finally, mix the paste primary product with water, heat and dissolve, then crystallize naturally, and collect it by filtration while cold.

[0052] This embodiment also provides a sterol, which is prepared by the above-mentioned method.

Embodiment 3

[0054] The present embodiment provides a kind of extraction method of sterol, it comprises the following steps:

[0055] First, after mixing 50mL seabuckthorn fruit oil with 350mL potassium hydroxide ethanol solution with a concentration of 0.4mol / L, saponification reaction was carried out at 81°C for 200min, and the saponified product was evaporated to remove ethanol at 35°C to obtain Saponification solution.

[0056] Next, take 400mL of the above saponified solution, add water to dilute to 1000mL, then extract 3 times with 400mL of petroleum ether, filter and mix the extracted products to obtain an extract, evaporate the extract at a temperature of 35°C, and evaporate the remaining The product was concentrated and then dried to obtain a paste-like primary product.

[0057] Finally, wash the paste-like primary product twice with 0.45mol / L potassium hydroxide, and then wash three times with distilled water, then heat and dissolve the solid in water, crystallize naturally, and...

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Abstract

The invention discloses an extraction method of sterol and sterol, and relates to the technical field of biochemical industry. The extraction method of the sterol comprises the following steps: enabling sea buckthorn oil to be subjected to a saponification reaction at the temperature of 75-81 DEG C so as to obtain a saponified solution; extracting the saponified solution by using petroleum ether, evaporating the separated extract at the temperature of 27-35 DEG C, and drying the residue obtained after evaporation so as to obtain a pasty primary product; dissolving the pasty primary product by heating, carrying out crystallization and filtering. The whole purification technology process is simple to operate and easy to implement, thus being suitable for industrial operation; furthermore, the extraction method is higher in safety coefficient and product purify, and has good application value. The invention also provides the sterol; the sterol is obtained by applying the extraction method, and is low in production cost and high in product purity.

Description

technical field [0001] The invention relates to the field of biochemical technology, and in particular to a method for extracting sterols and sterols. Background technique [0002] Nowadays, with the deepening of human understanding of sterols, especially sterols from natural plant sources, high-quality and efficient extraction of sterols has become a common goal of workers in various industries. Therefore, simple, efficient, practical and safe goals continue to promote the iteration of sterol extraction methods. The core of efficient utilization, after decades of development, the extraction methods of sterols have developed many methods such as solvent crystallization, complexation, molecular distillation, dry saponification, and enzymatic methods. [0003] However, the existing extraction method has the problems of low yield and high cost due to complicated purification process. Contents of the invention [0004] The object of the present invention is to provide a metho...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 李之越米世文龚晨何晓磊李甲云康忠军乔彬袁成萍孙园园乔岩陈诺
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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