Isolation and cloning of paddy rice photoperiod sensitive genic male sterility gene pms1 and application thereof

Abstract

The invention relates to isolation and cloning of paddy rice photoperiod sensitive genic male sterility gene pms1 and applications thereof. The pms1 gene has only one transcript PMS1T, which is a non-coding long-chain RNA. There are insertion/deletion variation as long as 65 bp and two mononucleotide mutations between different alleles of the gene, wherein the sequence of a dominant allele of the pms1 is represented as the SEQ ID No.4 and the sequence of a recessive allele is represented as the SEQ ID No.3. By means of the insertion/deletion variation and one mononucleotide mutation, two molecular markers of the paddy rice photoperiod sensitive genic male sterility gene pms1 are obtained. The dominant allele of the pms1 can reduce the setting rate of NIL (MH) under long-day condition, while excessive expression on total-length or shortcut PMS1T also can reduce the setting rate of NIL (MH) under long-day condition too, so that by inhibiting the expression of the PMS1T in a photoperiod sensitive genic male sterile line Nongken 58S, the fertility of the Nongken 58S can be recovered. The isolation and cloning of the gene has important utilization value for selective breeding and improvement of a novel paddy rice photoperiod sensitive genic male sterile line.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering. It specifically relates to the isolation and cloning, functional verification and application of a rice light-sensitive nuclear sterile gene pms1 in rice improvement. [0002] The pms1 gene isolated in the present invention has only one transcript, PMS1T, which is a non-coding long-chain RNA and does not code protein. There is a 65bp insertion / deletion variation and two single nucleotide mutations between different alleles of this gene. The sequence of the dominant allele of pms1 is shown in SEQ ID NO:4, and the sequence of the recessive allele is shown in SEQ ID NO:3. Using the insertion / deletion variation and a single nucleotide mutation, two molecular markers of rice photosensitive genic male sterile gene pms1 were obtained. The dominant allele of pms1 can reduce the seed setting rate of NIL(MH) under long day, but has no effect on the rate of NIL(MH) under short day. Overe...

AI Technical Summary

Problems solved by technology

The three-line hybrid rice technology is mature and widely planted in my country, but there are still some application limitations

Due to the single sterile cytoplasm of three-line hybrid rice, limited by the relationship between recovery and conservation, the combination of groups is not free enough, so the cycle of breeding new combinations is relatively long

In production, its breeding procedures and production links are more complicated, resulting in higher cost of seed production

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