Zearalenone fluorescent quantitative detection card and preparation method thereof
A fluorescence quantitative detection, zearalenone technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of long detection cycle, low sensitivity, complex operation, etc., to achieve fast detection speed, convenient operation, Guaranteed accuracy
Inactive Publication Date: 2017-12-01
普十生物科技(北京)有限公司
View PDF2 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
This assay method has disadvantages such as long detection period, complicated operation, low sensitivity, expensive equipment, etc., and cannot be applied to the screening of large batches of samples.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0024] The present invention will be further described below in combination with the drawings and specific embodiments.
[0025] The preparation method of the zearalenone fluorescence quantitative detection card is described in detail below. For the convenience of description, the abbreviations of some reagents used in the preparation method of the zearalenone fluorescence quantitative detection card are described as follows, and the corresponding reagents are abbreviated when describing the preparation method in detail.
[0026] 2-(N-morpholine)ethanesulfonic acid buffer, referred to as MES buffer;
[0027] 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, referred to as EDC;
[0028] N-hydroxysuccinimide, referred to as NHS;
[0029] 4-Hydroxyethylpiperazineethanesulfonic acid buffer, referred to as HEPES buffer;
[0030] Glycine, referred to as Gly;
[0031] Bovine serum albumin, referred to as BSA.
[0032] The preparation method of the zearalenone fluoresc...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
Login to View More
Abstract
The invention provides a zearalenone fluorescent quantitative detection card. The zearalenone fluorescent quantitative detection card comprises a bottom plate, and a sample pad, a glass fiber membrane, a nitrocellulose membrane and a water absorption pad which are arranged on the bottom plate and are lapped in sequence, wherein the glass fiber membrane is coated with zearalenone monoclonal antibody labeled by a fluorescent microsphere, detection lines and quality control lines are arranged on the nitrocellulose membrane at intervals, the detection lines are formed by coating the nitrocellulose membrane with zearalenone conjugate antigen, and the quality control lines are formed by coating the nitrocellulose membrane with goat anti mouse IgG. The zearalenone fluorescent quantitative detection card has the advantages of being simple in operation, high in flexibility, wide in linear range and the like. The invention also provides a preparation method of the zearalenone fluorescent quantitative detection card.
Description
【Technical field】 [0001] The invention relates to the technical field of biological detection, in particular to a zearalenone fluorescence quantitative detection card and a preparation method thereof. 【Background technique】 [0002] Zearalenone (Zearalenone), also known as F-2 toxin, is a metabolite of Gibberella zeae. It was first isolated from corn with scab. .tricinctum). Zearalenone mainly contaminates grains such as corn, wheat, rice, barley, millet and oats. Among them, the positive detection rate of corn is 45%, and the highest toxic content can reach 2909 mg / kg; the detection rate of wheat is 20%, and the toxic content is 0.364-11.05 mg / kg. The heat resistance of zearalenone is strong, and it can be completely destroyed after being treated at 110°C for 1 hour. [0003] Zearalenone has an estrogenic effect, mainly acting on the reproductive system, and can cause hyperestrogenism in livestock, poultry and experimental mice. Consumption of foods containing zearalen...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/64G01N33/577G01N33/558G01N33/533
CPCG01N33/64G01N33/533G01N33/558G01N33/577
Inventor 王琳魏照征闫俊杰
Owner 普十生物科技(北京)有限公司